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Literature summary for 7.2.2.10 extracted from
- Solubilization, purification and reconstitution of Ca2+-ATPase from bovine pulmonary artery smooth muscle microsomes by different detergents: Preservation of native structure and function of the enzyme by DHPC (2006), Biochim. Biophys. Acta, 1760, 20-31.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Bos taurus | 5783 | - |
| membrane | - |
Bos taurus | 16020 | - |
| microsome | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | Bos taurus | Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities | ADP + phosphate + Ca2+/trans | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from pulmonary artery smooth muscle microsomes using different detergents, best is 1,2-diheptanoyl-sn-phosphatidylcholine in a ratio of protein to detergent of 1,5:1, which preserves the native structure, conformational transition, and function of the enzyme, mechanism, overview, solubilization is followed by adsorption chromatography and gel filtration | Bos taurus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of purified enzyme into dioleoyl-phosphatidylcholine, in a buffer containing 50 mM MOPS, 0.25 M sucrose, 1 M KCl, 1 mM MgCl2, 0.1 mM CaCl2, 1 mM DTT, and 0.025% NaN3, at pH 7.5 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pulmonary artery | - |
Bos taurus | - |
| smooth muscle | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity during purification and after solubilization/reconstitution with different detergents, overview | Bos taurus |
| 4.07 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities | Bos taurus | ADP + phosphate + Ca2+/trans | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 115000, SDS-PAGE | Bos taurus |
| More | enzyme structure and conformational transition upon Ca2+ binding or release | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ca2+-ATPase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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