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Literature summary for 7.2.2.10 extracted from

  • Penheiter, A.R.; Filoteo, A.G.; Penniston, J.T.; Caride, A.J.
    Kinetic analysis of the calmodulin-binding region of the plasma membrane calcium pump isoform 4b (2005), Biochemistry, 44, 2009-2020.
    View publication on PubMed
Search for more literature for 7.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin the amino acid sequence of the calmodulin binding site is L1086RRGQILWFRGLNRIQTQIKVVKAFHSS1113, i.e. peptide 28, determination of essential residues for binding, overview, complex structure modelling, calmodulin binding mechanism, overview Homo sapiens
additional information 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length bindng site peptide 28 and truncated versions, overview, complex structure modelling, calmodulin binding mechanism, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
F1110A 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens
additional information 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens
V1107A 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding kinetics of calmodulin and labeled calmodulin to enzyme binding site-derived peptides, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Ca2+/cis Homo sapiens the enzyme is a plasma membrane calcium pump performing Ca2+-dependent ATPase and ATP-dependent Ca2+ transport reactions ADP + phosphate + Ca2+/trans
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform 4b
-

Purification (Commentary)

Purification (Comment) Organism
native PMCA4b from erythrocytes by calmodulin affiniyt chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte isoform 4b Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?
ATP + H2O + Ca2+/cis the enzyme is a plasma membrane calcium pump performing Ca2+-dependent ATPase and ATP-dependent Ca2+ transport reactions Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

Synonyms Comment Organism
PCMA4b
-
 Homo sapiens
plasma membrane calcium pump
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens