Literature summary for 7.2.2.10 extracted from

Osborn, K.D.; Zaidi, A.; Urbauer, R.J.; Michaelis, M.L.; Johnson, C.K.
Single-molecule characterization of the dynamics of calmodulin bound to oxidatively modified plasma-membrane Ca2+-ATPase (2005), Biochemistry, 44, 11074-11081.

Search for more literature for 7.2.2.10

Activating Compound

Activating Compound	Comment	Organism	Structure
Calmodulin	activates, single-molecule characterization of the dynamics of calmodulin bound to oxidatively modified PMCA, overview	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme has an autoinhibitory domain which is coupled to the ATP binding domain, oxidative modification alters the interaction and inhibits the enzyme, proteolysis pattern of inactive PMCAox by chymotrypsin, overview	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	the enzyme has 10 transmembrane helices	Homo sapiens	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required, activates the ATP hydrolysis activity	Homo sapiens
Mg2+	required	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
138000	-	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Ca2+/cis	Homo sapiens	-	ADP + phosphate + Ca2+/trans	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Oxidation Stability

Oxidation Stability	Organism
oxidative moculation of the enzyme leads to loss of activity due to alterations of the coupling between the ATP binding domain and the autoinhibitory domain, proteolysis pattern of PMCAox by chymotrypsin, overview	Homo sapiens

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	chymotrypsin has a cleavage site in the autoinhibitory domain of the native 138 kDa enzyme resultng in a 125 kDa protein, proteolysis pattern of inactive PMCAox by chymotrypsin	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from blood by calmodulin affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+/cis	-	Homo sapiens	ADP + phosphate + Ca2+/trans	-	?

Synonyms

Synonyms	Comment	Organism
plasma-membrane Ca2+-ATPase	-	Homo sapiens
PMCA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)