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Literature summary for 7.2.2.10 extracted from

  • Mahaney, J.E.; Thomas, D.D.; Froehlich, J.P.
    The time-dependent distribution of phosphorylated intermediates in native sarcoplasmic reticulum Ca2+-ATPase from skeletal muscle is not compatible with a linear kinetic model (2004), Biochemistry, 43, 4400-4416.
    View publication on PubMed
Search for more literature for 7.2.2.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed de-/phosphorylation reaction kinetics, kinetic modelling Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Oryctolagus cuniculus 16020
-
sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Ca2+/cis Oryctolagus cuniculus the enzyme is the Ca2+ pump in sarcoplasmic reticulum membranes, the ATP hydrolysis energy is used for uphill transport and accumulation of Ca2+ ADP + phosphate + Ca2+/trans
-
 r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 New Zealand rabbits
-

Purification (Commentary)

Purification (Comment) Organism
partially, preparation of sarcoplasmic reticulum membrane vesicles Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2] reaction and kinetic mechanism, phosphorylated intermediate, detailed overview Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle fast twitch skeletal muscle Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis the enzyme is the Ca2+ pump in sarcoplasmic reticulum membranes, the ATP hydrolysis energy is used for uphill transport and accumulation of Ca2+ Oryctolagus cuniculus ADP + phosphate + Ca2+/trans
-
 r
ATP + H2O + Ca2+/cis assay with labeled ATP substrate Oryctolagus cuniculus ADP + phosphate + Ca2+/trans
-
 r
additional information the enzyme performs ATP-dependent Ca2+ transport and Ca2+-dependent ATPase activity, the detergent-solubilized enzyme shows monomeric catalytic function as deduced from kinetic modelling, while the native enzyme shows features of oligomeric protein conformational interactions that constrain the subunits to a staggered or out-of-phase mode of action Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
More the detergent-solubilized enzyme shows monomeric catalytic function as deduced from kinetic modelling, while the native enzyme shows features of oligomeric protein conformational interactions that constrain the subunits to a staggered or out-of-phase mode of action Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
adenosinetriphosphatase
-
 Oryctolagus cuniculus
Ca-ATPase
-
 Oryctolagus cuniculus
SERCA1
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2 21 assay at 2°C and at 21°C Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Oryctolagus cuniculus