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Literature summary for 7.2.2.10 extracted from

  • Lenoir, G.; Picard, M.; Moller, J.V.; le Maire, M.; Champeil, P.; Falson, P.
    Involvement of the L6-7 loop in SERCA1a Ca2+-ATPase activation by Ca2+ (or Sr2+) and ATP (2004), J. Biol. Chem., 279, 32125-32133.
    View publication on PubMed
Search for more literature for 7.2.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild type and mutant D813A/D818A of L6-7 loop of enzyme in yeast Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
D813A/D818A the mutant displays a very low activity in presence of detergent, but the same maximal velocity and apparent affinity for Ca2+ as the wild-type enzyme in absence of detergent, the mutation affects pronotation-dependent winding and unwinding events in the nearby M6 transmembrane segment Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
detergent C12E8 inhibition of mutant D813A/D818A Oryctolagus cuniculus
orthovanadate
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 Ca2+ wild-type enzyme in absence of detergent Oryctolagus cuniculus
0.001 0.002 Ca2+ mutant D813A/D818A in absence of detergent Oryctolagus cuniculus
0.015
-
 Sr2+ mutant D813A/D818A in absence of detergent Oryctolagus cuniculus
0.035
-
 Sr2+ wild-type enzyme in absence of detergent Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Oryctolagus cuniculus 16020
-
sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type and mutant D813A/D818A of the L6-7 loop of enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis
-
 Oryctolagus cuniculus ADP + phosphate + Ca2+/trans
-
 ?
ATP + Sr2+/cis + H2O
-
 Oryctolagus cuniculus ADP + phosphate + Sr2+/trans
-
 ?

Synonyms

Synonyms Comment Organism
SERCA1a
-
 Oryctolagus cuniculus