Literature summary for 7.2.2.1 extracted from

Yamamoto, M.; Unzai, S.; Saijo, S.; Ito, K.; Mizutani, K.; Suno-Ikeda, C.; Yabuki-Miyata, Y.; Terada, T.; Toyama, M.; Shirouzu, M.; Kobayashi, T.; Kakinuma, Y.; Yamato, I.; Yokoyama, S.; Iwata, S.; Murata, T.
Interaction and stoichiometry of the peripheral stalk subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI of Enterococcus hirae V-ATPase (2008), J. Biol. Chem., 283, 19422-19431.

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Cloned(Commentary)

Cloned (Comment)	Organism
genes ntpE and ntpF encoding the two subunits of the enzyme, expression of both subunits and the N-terminal soluble domain of NtpI as His-tagged proteins in Escherichia coli strain BL21(DE3)	Enterococcus hirae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuolar membrane	the enzyme is orientated to the cytoplasmic side, structural model of V-ATPase, overview	Enterococcus hirae	5774	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Na+/in	Enterococcus hirae	-	ADP + phosphate + Na+/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Enterococcus hirae	-	genes ntpE and ntpF	-

Purification (Commentary)

Purification (Comment)	Organism
separate purification of His-tagged NtpE and NtpF, and His-tagged N-terminal hydrophilic domain of NtpI by nickel affinity and ion exchange chromatography, and gel filtration	Enterococcus hirae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Na+/in	-	Enterococcus hirae	ADP + phosphate + Na+/out	-	?

Subunits

Subunits	Comment	Organism
More	the peripheral stalk of Na+-translocating V-ATPase of Enterococcus hirae is composed of NtpE and NtpF subunits together with the N-terminal hydrophilic domain of NtpI, structural model of V-ATPase, overview. Subunit interaction analysis by pulldown assay and surface plasmon resonance, detailed overview	Enterococcus hirae

Synonyms

Synonyms	Comment	Organism
Na+-translocating V-ATPase	-	Enterococcus hirae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Enterococcus hirae

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Release 2023.1 (January 2023)