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Literature summary for 7.2.2.1 extracted from

  • Muller, V.; Aufurth, S.; Rahlfs, S.
    The Na(+) cycle in Acetobacterium woodii: identification and characterization of a Na(+) translocating F(1)F(0)-ATPase with a mixed oligomer of 8 and 16 kDa proteolipids (2001), Biochim. Biophys. Acta, 1505, 108-120.
    View publication on PubMed
Search for more literature for 7.2.2.1

Inhibitors

Inhibitors Comment Organism Structure
5-(N,N-Hexamethylene)amiloride 70% inhibition at 0.1 mM, Na+ protects against inactivation Acetobacterium woodii
5-(N-ethyl-N-isopropyl)amiloride 70% inhibition at 0.1 mM, Na+ protects against inactivation Acetobacterium woodii
azide inhibition of both ATPase and Na+ translocation activities Acetobacterium woodii
diethylstilbestrol 30% inhibition at 0.001 mM Acetobacterium woodii
additional information not inhibited by vanadate Acetobacterium woodii
N,N'-dicyclohexylcarbodiimide inhibition of both ATPase and Na+ translocation activities, complete inhibition at 0.005 mM Acetobacterium woodii
N-10-benzylamiloride 70% inhibition at 0.1 mM, Na+ protects against inactivation Acetobacterium woodii
NO3- 50% inhibition at 10 mM Acetobacterium woodii
tributyltin 85% inhibition at 0.001 mM Acetobacterium woodii
Venturicidin inhibition of both ATPase and Na+ translocation activities Acetobacterium woodii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 ATP
-
 Acetobacterium woodii
0.4
-
 Na+/out pH 7.5 Acetobacterium woodii

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 75% of the maximal activity compared to saturating Na+ concentrations Acetobacterium woodii
Mg2+ necessary for ATPase activity Acetobacterium woodii
SO32-
-
 Acetobacterium woodii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii
19000
-
 ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii
35000
-
 ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii
52000
-
 ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii
57000
-
 ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii
590000
-
 gel filtration Acetobacterium woodii

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
 homoacetogenic Gram-positive bacterium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + Na+/out
-
 Acetobacterium woodii ATP + H2O + Na+/in
-
 r
CDP + phosphate + Na+/out lower activity compared to ADP Acetobacterium woodii CTP + H2O + Na+/in
-
 r
GDP + phosphate + Na+/out lower activity compared to ADP Acetobacterium woodii GTP + H2O + Na+/in
-
 r
IDP + phosphate + Na+/out lower activity compared to ADP Acetobacterium woodii ITP + H2O + Na+/in
-
 r
UDP + phosphate + Na+/out lower activity compared to ADP Acetobacterium woodii UTP + H2O + Na+/in
-
 r

Subunits

Subunits Comment Organism
oligomer ? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE Acetobacterium woodii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 at 0.1 mM of Na+ Acetobacterium woodii
7.5 9 at 5 mM of Na+ Acetobacterium woodii