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Literature summary for 7.2.2.1 extracted from
- Purification and characterization of the catalytic moiety of vacuolar-type Na+-ATPase from Enterococcus hirae (1994), J. Biochem., 116, 1302-1308.
General Stability
| General Stability | Organism |
|---|---|
| without glycerol, the purified enzyme, 1 mg/ml in 50 mM Tris-HCl, pH 7.5 is inactivated by overnight exposure to 4°C | Enterococcus hirae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Enterococcus hirae |  |
| N,N'-dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| NEM | - |
Enterococcus hirae | |
| NO3- | - |
Enterococcus hirae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Enterococcus hirae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | no stimulation by Na+ | Enterococcus hirae | |
| Na+ | the enzyme has Na+-translocating activity | Enterococcus hirae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 29000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 52000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 400000 | - |
gel filtration | Enterococcus hirae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus hirae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus hirae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.73 | - |
- |
Enterococcus hirae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | ADP + phosphate | - |
? | |
| CTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | CDP + H2O | - |
? | |
| GTP + H2O | Na+-translocating activity, GTP is hydrolyzed as the best substrate | Enterococcus hirae | GDP + phosphate | - |
? | |
| UTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | UDP + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Enterococcus hirae |
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