General Stability | Organism |
---|---|
without glycerol, the purified enzyme, 1 mg/ml in 50 mM Tris-HCl, pH 7.5 is inactivated by overnight exposure to 4°C | Enterococcus hirae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Enterococcus hirae | |
N,N'-dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
NEM | - |
Enterococcus hirae | |
NO3- | - |
Enterococcus hirae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Enterococcus hirae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | no stimulation by Na+ | Enterococcus hirae | |
Na+ | the enzyme has Na+-translocating activity | Enterococcus hirae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
29000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
52000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
400000 | - |
gel filtration | Enterococcus hirae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus hirae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus hirae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.73 | - |
- |
Enterococcus hirae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | ADP + phosphate | - |
? | |
CTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | CDP + H2O | - |
? | |
GTP + H2O | Na+-translocating activity, GTP is hydrolyzed as the best substrate | Enterococcus hirae | GDP + phosphate | - |
? | |
UTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | UDP + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Enterococcus hirae |