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Literature summary for 7.2.2.1 extracted from
- Purification and reconstitution of Na+-translocating vacuolar ATPase from Enterococcus hirae (1997), J. Biol. Chem., 272, 24885-24890.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Enterococcus hirae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| destruxin B | - |
Enterococcus hirae |  |
| N,N'-dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| NEM | - |
Enterococcus hirae | |
| NO3- | KNO3- | Enterococcus hirae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
ATP | - |
Enterococcus hirae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Enterococcus hirae | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | enzyme activity is strictly dependent on the presence of Na+ or Li+ ions | Enterococcus hirae | |
| Li+ | translocation of Li+ | Enterococcus hirae | |
| Na+ | the enzyme has Na+-translocating activity | Enterococcus hirae | |
| Na+ | enzyme activity is strictly dependent on the presence of Na+ or Li+ ions | Enterococcus hirae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 14000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 15000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 24000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 27000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 38000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 52000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 65000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 69000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus hirae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus hirae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, solubilized enzyme is stable for at least 3 months | Enterococcus hirae |
| 4°C, solubilized enzyme is stable for at least 1 week | Enterococcus hirae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | translocation of Li+ | Enterococcus hirae | ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
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