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Literature summary for 7.2.1.3 extracted from

  • Su D., Asard H.
    Three mammalian cytochromes b561 are ascorbate-dependent ferrireductases (2006), FEBS J., 273, 3722-3374 .
    View publication on PubMed
Search for more literature for 7.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a Saccharomyces cerevisiae DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity Mus musculus

Protein Variants

Protein Variants Comment Organism
D38A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
E177A the mutation of lysosomal cytochrome b561 reduces the enzyme activity compared to the wild type Mus musculus
E196A the mutation of lysosomal cytochrome b561 strongly reduces the enzyme activity compared to the wild type Mus musculus
F44A the mutation of lysosomal cytochrome b561 reduces the enzyme activity by about 45% compared to the wild type Mus musculus
H105A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
H112A the mutation of lysosomal cytochrome b561 reduces the enzyme activity compared to the wild type Mus musculus
H117A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
H156A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
H47A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
H83A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
M51A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
N106A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
P48A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
Q131A the mutation of lysosomal cytochrome b561 reduces the enzyme activity to 45% of that of the wild type Mus musculus
R149A the mutation of lysosomal cytochrome b561 results in a 75% loss in activity compared to the wild type enzyme Mus musculus
R67A the mutation of lysosomal cytochrome b561 results in an almost complete loss of the FeCN reductase activity of the enzyme Mus musculus
S115A the ferrireductase activity of lysosomal cytochrome b561 in mutant S115A is reduced to 50% compared to the wild type Mus musculus
S118A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
W119A the ferrireductase activity of lysosomal cytochrome b561 in mutant W119A is reduced to 17% compared to the wild type Mus musculus
Y190A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
Y66A the mutation of lysosomal cytochrome b561 results in an almost complete loss of the FeCN reductase activity of the enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane a transmembrane protein Mus musculus 5886
-

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
duodenum duodenal cytochrome b561 Mus musculus
-
kidney chromaffin granule cytochrome b561 and lysosomal cytochrome b561 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ascorbate[side 1] + Fe3+-EDTA[side 2] compared with FeCN, Fe3+-EDTA is a relatively poor substrate for the enzyme (20-35fold lower ferrireductase activities) Mus musculus monodehydroascorbate[side 1] + Fe2+-EDTA[side 2]
-
 ?
ascorbate[side 1] + ferricyanide[side 2]
-
 Mus musculus monodehydroascorbate[side 1] + ferrocyanide[side2]
-
-

Subunits

Subunits Comment Organism
? x * 27000, lysosomal cytochrome b561, SDS-PAGE Mus musculus
? x * 27800, chromaffin granule cytochrome b561, SDS-PAGE Mus musculus
? x * 31500, duodenal cytochrome b561, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
ascorbate-dependent ferrireductase
-
 Mus musculus
CGCytb
-
 Mus musculus
chromaffin granule cytochrome b561
-
 Mus musculus
Cyt b561
-
 Mus musculus
cytochrome b561
-
 Mus musculus
DCytb
-
 Mus musculus
duodenal cytochrome b561
-
 Mus musculus
LCytb
-
 Mus musculus
lysosomal cytochrome b561
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ascorbate the enzyme is dependent on the presence of intracellular ascorbate Mus musculus
heme a di-heme enzyme Mus musculus