BRENDA - Enzyme Database
show all sequences of 7.2.1.2

Reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by NAD+ catalyzed by ferredoxin-NAD+ reductase (Rnf)

Chowdhury, N.P.; Klomann, K.; Seubert, A.; Buckel, W.; J. Biol. Chem. 291, 11993-12002 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acidaminococcus fermentans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00026
-
reduced ferredoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
0.00042
-
reduced flavodoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Acidaminococcus fermentans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Na+
required for activity
Acidaminococcus fermentans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans DSM 20731
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans VR4
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidaminococcus fermentans
-
-
-
Acidaminococcus fermentans VR4
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
-
Acidaminococcus fermentans
NAD+
-
Acidaminococcus fermentans
[4Fe-4S]-center
-
Acidaminococcus fermentans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acidaminococcus fermentans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
-
Acidaminococcus fermentans
NAD+
-
Acidaminococcus fermentans
[4Fe-4S]-center
-
Acidaminococcus fermentans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00026
-
reduced ferredoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
0.00042
-
reduced flavodoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Acidaminococcus fermentans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Na+
required for activity
Acidaminococcus fermentans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans DSM 20731
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans VR4
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
Other publictions for EC 7.2.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745340
Chowdhury
Reduction of flavodoxin by el ...
Acidaminococcus fermentans, Acidaminococcus fermentans VR4
J. Biol. Chem.
291
11993-12002
2016
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1
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1
1
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3
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2
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6
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3
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1
3
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2
1
1
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3
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6
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-
741690
Lang
New mode of energy metabolism ...
Candidatus Methanoplasma termitum, Candidatus Methanoplasma termitum MpT1
Appl. Environ. Microbiol.
81
1338-1352
2015
-
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1
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1
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10
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1
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1
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1
1
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-
727962
Hess
The ferredoxin: NAD+ oxidoredu ...
Acetobacterium woodii, Acetobacterium woodii DSM 1030
J. Biol. Chem.
288
31496-31502
2013
3
-
-
-
-
-
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3
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3
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2
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6
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1
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2
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3
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3
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3
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6
-
1
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-
2
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-
-
-
1
1
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-
-
733825
Schlegel
Electron transport during acet ...
Methanosarcina acetivorans
FEBS J.
279
4444-4452
2012
1
-
-
-
-
-
-
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1
1
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1
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3
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3
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1
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2
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1
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2
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1
1
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1
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3
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-
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1
-
-
-
-
2
2
-
-
-
732808
Biegel
Bacterial Na+-translocating fe ...
Acetobacterium woodii
Proc. Natl. Acad. Sci. USA
107
18138-18142
2010
-
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-
-
-
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6
1
1
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1
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5
-
-
-
-
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-
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2
-
1
-
-
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1
-
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2
-
-
5
-
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-
2
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5
6
-
1
1
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1
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2
-
1
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1
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1
1
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-
-
731723
Biegel
Genetic, immunological and bio ...
Acetobacterium woodii
Environ. Microbiol.
11
1438-1443
2009
-
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1
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-
-
-
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1
-
5
-
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4
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1
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-
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1
1
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-
-
-
-
1
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-
1
1
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-
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-
-
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1
-
5
-
-
-
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1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
733377
Schmidt
The ins and outs of Na+ bioene ...
Acetobacterium woodii
Biochim. Biophys. Acta
1787
691-696
2009
-
-
-
-
-
-
-
-
1
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
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2
-
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-
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1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
733098
Mueller
Discovery of a ferredoxin:NAD+ ...
Acetobacterium woodii, Acetobacterium woodii DSM 1030, Azotobacter vinelandii, Clostridium tetani, Clostridium tetanomorphum, Methanosarcina acetivorans, Rhodobacter capsulatus, Syntrophus aciditrophicus
Ann. N. Y. Acad. Sci.
1125
137-146
2008
-
-
2
-
-
-
-
-
3
7
-
8
-
10
-
-
5
-
-
-
-
-
11
5
-
-
-
-
-
-
-
27
-
-
-
-
-
2
27
-
-
-
-
-
-
-
3
7
-
8
-
-
-
5
-
-
-
-
11
5
-
-
-
-
-
-
-
-
-
10
10
-
-
-
733325
Backiel
Covalent binding of flavins to ...
Vibrio cholerae, Vibrio cholerae O395N-1
Biochemistry
47
11273-11284
2008
-
-
1
-
3
-
-
-
1
1
2
2
-
3
-
-
1
-
-
-
-
-
6
2
-
-
-
-
-
-
-
5
-
-
-
-
-
1
5
-
3
-
-
-
-
-
1
1
2
2
-
-
-
1
-
-
-
-
6
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-