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Literature summary for 7.2.1.2 extracted from

  • Hess, V.; Schuchmann, K.; Müller, V.
    The ferredoxin: NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium woodii requires Na+ and is reversibly coupled to the membrane potential (2013), J. Biol. Chem., 288, 31496-31502.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ETH2120 reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 155% of control Acetobacterium woodii
monensin reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 138% of control Acetobacterium woodii
valinomycin reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 128% of control Acetobacterium woodii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
NAD+ pH 7.7, 30°C Acetobacterium woodii
0.155
-
Na+/in pH 6, 30°C Acetobacterium woodii
0.201
-
Na+/in pH 7.7, 30°C Acetobacterium woodii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii DSM 103 electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii DSM 1030 electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
-
-
Acetobacterium woodii DSM 1030
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii DSM 103 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii DSM 103 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii DSM 1030 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii DSM 1030 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?

Synonyms

Synonyms Comment Organism
reduced ferredoxin:NAD+ oxidoreductase
-
Acetobacterium woodii
Rnf complex
-
Acetobacterium woodii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acetobacterium woodii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Acetobacterium woodii
7.7
-
assay at Acetobacterium woodii

General Information

General Information Comment Organism
metabolism electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii