Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.1.1 extracted from

  • Casutt, M.S.; Nedielkov, R.; Wendelspiess, S.; Vossler, S.; Gerken, U.; Murai, M.; Miyoshi, H.; Moeller, H.M.; Steuber, J.
    Localization of ubiquinone-8 in the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae (2011), J. Biol. Chem., 286, 40075-40082.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length enzyme and N-terminally His6-tagged large subunit NqrA in Vibrio cholerae strain O395 N1 DELTAnqr Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
2-n-heptyl-4-hydroxyquinoline N-oxide
-
 Vibrio cholerae serotype O1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-embedded Vibrio cholerae serotype O1 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains one FeS cluster Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50793
-
 1 * 50793, subunit NqrA, mass spectrometry Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
 NAD+ + ubiquinol + n Na+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged monomeric large subunit NqrA from Vibrio cholerae strain O395 N1 DELTAnqr membranes by ultracentrifugation, nickel affinity chromatography, and gel filtration in the presence of 0.05% w/v n-dodecyl-beta-D-maltoside Vibrio cholerae serotype O1

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of recombinant Na+-NQR and recombinant His6-tagged NqrA with ubiquinone-8, overview Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information binding activity of subunit NqrA with different quinones, i.e. quinone, ubiquinone-1, ubiquinone-2, ubiquinone-8, 3-azido-2-methoxy-5-methyl-6-geranyl-1,4-benzoquinone, 2-azido-3-methoxy-5-methyl-6-geranyl-1,4-benzoquinone, 3-azido-2-methoxy-5-methyl-6-geranyl-1,4-benzoquinone-biotin, biotinylated 3-azido-2-methoxy-5-methyl-6-geranyl-1,4-benzoquinone, overview Vibrio cholerae serotype O1 ?
-
 ?
NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?
NADH + H+ + ubiquinone + n Na+/in the large, peripheral NqrA subunit of the Na+-NQR binds one molecule of ubiquinone-8 with high affinity, which is determined by the methoxy groups at the C-2 and C-3 positions of the quinone headgroup. Ubiquinone-8 bound to NqrA occupies a functional site. Analysis of dynamic interaction of NqrA with quinones by surface plasmon resonance and saturation transfer difference NMR. Electron transfer in Na+-NQR is initiated by NADH oxidation on subunit NqrF and leads to quinol formation on subunit NqrA Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?

Subunits

Subunits Comment Organism
hexamer Na+-NQR is a multisubunit, membrane-embedded NADH dehydrogenase Vibrio cholerae serotype O1
monomer 1 * 50793, subunit NqrA, mass spectrometry Vibrio cholerae serotype O1
More putative arrangement of subunits and cofactors of the Na+-NQR Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
Na+-NQR
-
 Vibrio cholerae serotype O1
Na+-pumping NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
flavin the enzyme contains four flavins Vibrio cholerae serotype O1
additional information putative arrangement of subunits and cofactors of the Na+-NQR Vibrio cholerae serotype O1
NADH
-
 Vibrio cholerae serotype O1

General Information

General Information Comment Organism
physiological function Vibrio cholerae relies on the Na+-pumping NADH:quinone oxidoreductase as the first complex in its respiratory chain Vibrio cholerae serotype O1