BRENDA - Enzyme Database
show all sequences of 7.2.1.1

Energy transducing redox steps of the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae

Juarez, O.; Morgan, J.E.; Nilges, M.J.; Barquera, B.; Proc. Natl. Acad. Sci. USA 107, 12505-12510 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C76A
mutant of subunit NqrF which lacks the 2Fe-2S-center cofactor and shows a 90% decrease in menadione reductase activity
Vibrio cholerae
D346A
the redox reaction in this mutant is significantly inhibited and the Na+ sensitivity of CoQ reduction is completely abolished, while the apparent affinity for Na+ is essentially unchanged. In D346A, electron transfer between FMN from subunit NqrB and riboflavin is inhibited
Vibrio cholerae
D397A
the reduction of subunit NqrB mutant D397A by NADH is very similar to the reduction of wild type enzyme but does not show sensitivity to Na+, and the rate of the stable neutral riboflavin radical to riboflavin-H2 is decreased 3fold. In NqrB-D397A, the reaction takes place in two steps: rapid oxidation of riboflavin and the two FMNs, followed by oxidation of FAD, at a 16fold lower rate
Vibrio cholerae
T225Y
the mutant lacks FMN from subunit NqrC and is unable to produce an electrochemical potential under either partial or multiple turnover conditions
Vibrio cholerae
T236Y
the mutant lacks FMN from subunit NqrB and is unable to produce an electrochemical potential under either partial or multiple turnover conditions
Vibrio cholerae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio cholerae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADH + H+ + ubiquinone + n Na+/in
-
713414
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains one noncovalently bound FAD
Vibrio cholerae
FMN
contains two FMNs covalently bound to the NqrB and NqrC subunits
Vibrio cholerae
NADH
-
Vibrio cholerae
riboflavin
contains one noncovalently bound riboflavin
Vibrio cholerae
[2Fe-2S]-center
contains one 2Fe-2S-center
Vibrio cholerae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains one noncovalently bound FAD
Vibrio cholerae
FMN
contains two FMNs covalently bound to the NqrB and NqrC subunits
Vibrio cholerae
NADH
-
Vibrio cholerae
riboflavin
contains one noncovalently bound riboflavin
Vibrio cholerae
[2Fe-2S]-center
contains one 2Fe-2S-center
Vibrio cholerae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C76A
mutant of subunit NqrF which lacks the 2Fe-2S-center cofactor and shows a 90% decrease in menadione reductase activity
Vibrio cholerae
D346A
the redox reaction in this mutant is significantly inhibited and the Na+ sensitivity of CoQ reduction is completely abolished, while the apparent affinity for Na+ is essentially unchanged. In D346A, electron transfer between FMN from subunit NqrB and riboflavin is inhibited
Vibrio cholerae
D397A
the reduction of subunit NqrB mutant D397A by NADH is very similar to the reduction of wild type enzyme but does not show sensitivity to Na+, and the rate of the stable neutral riboflavin radical to riboflavin-H2 is decreased 3fold. In NqrB-D397A, the reaction takes place in two steps: rapid oxidation of riboflavin and the two FMNs, followed by oxidation of FAD, at a 16fold lower rate
Vibrio cholerae
T225Y
the mutant lacks FMN from subunit NqrC and is unable to produce an electrochemical potential under either partial or multiple turnover conditions
Vibrio cholerae
T236Y
the mutant lacks FMN from subunit NqrB and is unable to produce an electrochemical potential under either partial or multiple turnover conditions
Vibrio cholerae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADH + H+ + ubiquinone + n Na+/in
-
713414
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
Na+-NQR couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. Na+ uptake by Na+-NQR is a nonelectrogenic process that occurs upon electron transfer from the 2Fe-2S-center to FMN of subunit NqrC, while the electrogenic transport of Na+ across the membrane, and likely release into solution, occurs upon electron transfer from FMN of subunit NqrB to riboflavin
Vibrio cholerae
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Na+-NQR couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. Na+ uptake by Na+-NQR is a nonelectrogenic process that occurs upon electron transfer from the 2Fe-2S-center to FMN of subunit NqrC, while the electrogenic transport of Na+ across the membrane, and likely release into solution, occurs upon electron transfer from FMN of subunit NqrB to riboflavin
Vibrio cholerae
Other publictions for EC 7.2.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742030
Toulouse
Strong pH dependence of coupl ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
Biol. Chem.
398
251-260
2017
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742911
Ito
-
Identification of the binding ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
J. Biol. Chem.
292
7727-7742
2017
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5
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5
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741981
Belevich
Identification of the couplin ...
Vibrio harveyi
Biochim. Biophys. Acta
1857
141-149
2016
1
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1
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4
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741985
Vorburger
Role of the Na+-translocating ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39541
Biochim. Biophys. Acta
1857
473-482
2016
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1
1
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742791
Muras
The Na+-translocating NADHqui ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
J. Bacteriol.
198
2307-2317
2016
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1
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7
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1
1
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732678
Borshchevskiy
Structural and functional inve ...
Vibrio harveyi, Vibrio harveyi CAIM 1792
PLoS ONE
10
e0118548
2015
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1
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4
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742029
Steuber
The structure of Na+-transloc ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
Biol. Chem.
396
1015-1030
2015
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1
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5
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732791
Minato
Roles of the sodium-translocat ...
Vibrio cholerae
PLoS ONE
9
e97083
2014
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741533
Vohl
-
Crystallization and prelimina ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39541
Acta Crystallogr. Sect. F
70
987-992
2014
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741955
Bertsova
Localization-controlled speci ...
Klebsiella pneumoniae
Biochim. Biophys. Acta
1837
1122-1129
2014
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742860
Strickland
The conformational changes in ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39541
J. Biol. Chem.
289
23723-23733
2014
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743239
Minato
-
Inhibition of the sodium-tran ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39541
Microb. Pathog.
66
36-39
2014
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743346
Steuber
Structure of the V. cholerae ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
Nature
516
62-67
2014
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1
1
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8
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725538
Nedielkov
NMR reveals double occupancy o ...
Vibrio cholerae
J. Biol. Chem.
288
30597-30606
2013
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1
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3
3
3
1
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725539
Shea
Aspartic acid 397 in subunit B ...
Vibrio cholerae, Vibrio cholerae ATCC 39541
J. Biol. Chem.
288
31241-31249
2013
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1
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7
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12
1
3
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2
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741884
Neehaul
-
Infrared spectroscopic eviden ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39541
Biochemistry
52
3085-3093
2013
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742842
Bertsova
Alternative pyrimidine biosyn ...
Klebsiella pneumoniae 204, Klebsiella pneumoniae, Vibrio harveyi, Vibrio harveyi R3
J. Biol. Chem.
288
14276-14286
2013
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3
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725481
Juarez
The role of glycine residues 1 ...
Vibrio cholerae, Vibrio cholerae ATCC 39541
J. Biol. Chem.
287
25678-25685
2012
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11
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11
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10
1
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3
3
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1
1
724251
Fadeeva
Cys377 residue in NqrF subunit ...
Vibrio harveyi
Biochemistry (Moscow)
76
186-195
2011
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1
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8
2
1
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4
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1
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1
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1
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725433
Juarez
The role and specificity of th ...
Vibrio cholerae
J. Biol. Chem.
286
26383-26390
2011
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1
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725448
Casutt
Localization of ubiquinone-8 i ...
Vibrio cholerae
J. Biol. Chem.
286
40075-40082
2011
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1
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1
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1
1
1
1
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4
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3
1
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1
3
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1
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1
1
1
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1
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3
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Sodium-translocating NADH:quin ...
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5
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5
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1
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1
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1
4
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1
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4
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2
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4
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5
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1
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1
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1
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700061
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1
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1
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6
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1
1
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1
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1
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1
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1
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1
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696157
Fadeeva
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4
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2
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696386
Tao
Oxidant-induced formation of a ...
Vibrio cholerae
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4
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1
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2
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1
2
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1
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5
2
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-
1
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-
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4
2
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1
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697973
Fadeeva
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279
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5
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3
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3
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3
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-
3
12
-
3
-
-
-
2
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3
-
-
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5
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698842
Juarez
Riboflavin is an active redox ...
Vibrio cholerae
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2
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1
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1
-
2
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-
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-
-
-
-
-
-
-
-
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699385
Tao
The Na+-translocating NADH:qui ...
Vibrio cholerae
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2008
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1
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1
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-
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-
2
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-
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2
-
1
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1
1
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-
1
-
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712274
Lin
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2
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2
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-
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6
-
-
1
-
-
-
6
-
-
-
1
2
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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671141
Tao
Crystallization of the NADH-ox ...
Vibrio cholerae
Acta Crystallogr. Sect. F
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2006
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1
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1
1
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3
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1
1
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-
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3
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3
1
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1
1
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1
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1
1
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-
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671351
Brandt
Energy converting NADH:quinone ...
Arabidopsis thaliana, Bos taurus, Caenorhabditis elegans, Chlamydomonas reinhardtii, Drosophila melanogaster, Escherichia coli, Homo sapiens, Klebsiella pneumoniae, Mus musculus, Neurospora crassa, Oryza sativa, Paracoccus denitrificans, Rhodomonas salina, Thermus thermophilus, Yarrowia lipolytica
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15
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45
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15
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45
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93
-
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15
15
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42
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60
15
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672135
Bogachev
Thermodynamic properties of th ...
Vibrio harveyi
Biochemistry
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1
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-
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2
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-
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1
2
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1
1
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1
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1
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Specific modification of a Na+ ...
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2
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2
1
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1
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1
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1
2
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1
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2
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2
1
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674705
Barquera
A new flavin radical signal in ...
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2
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1
1
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2
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2
-
2
-
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-
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1
1
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1
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1
1
-
-
-
-
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-
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-
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672293
Bogachev
Na(+)-translocating NADH:quino ...
Haemophilus influenzae, Klebsiella pneumoniae, Neisseria gonorrhoeae, Neisseria meningitidis, Porphyromonas gingivalis, Pseudomonas aeruginosa, Salinivibrio costicola, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio harveyi, Yersinia pestis
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33
1
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33
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34
1
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14
6
-
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11
1
-
-
-
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-
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674443
Lin
A vertebrate-type ferredoxin d ...
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22560-22563
2005
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-
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1
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5
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-
-
-
-
-
-
-
-
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-
1
-
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-
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-
1
-
-
-
-
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-
-
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1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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711269
Bogachev
Na+-translocating NADH:quinone ...
Haemophilus influenzae, Klebsiella pneumoniae, Neisseria gonorrhoeae, Neisseria meningitidis, Porphyromonas gingivalis, Pseudomonas aeruginosa, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio harveyi, Yersinia pestis
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22
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22
11
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66
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66
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22
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11
6
22
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22
11
-
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658029
Barquera
Mutagenesis study of the 2Fe-2 ...
Vibrio cholerae, Vibrio cholerae ATCC 39315
Biochemistry
43
12322-12330
2004
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1
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7
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1
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2
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8
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1
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5
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5
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1
9
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13
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1
-
2
-
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1
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-
-
5
-
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-
-
-
-
-
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659342
Trk
NADH oxidation by the Na+-tran ...
Vibrio cholerae, Vibrio cholerae ATCC 39315
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279
21349-21355
2004
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1
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-
-
-
1
-
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-
-
-
-
-
2
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
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-
-
-
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658968
Barquera
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1
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1
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-
1
-
-
-
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-
1
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
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-
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1
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392715
Barquera
Purification and characterizat ...
Vibrio cholerae
Biochemistry
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1
1
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1
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-
3
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-
-
-
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4
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-
-
-
-
1
4
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-
-
-
-
-
-
1
1
1
1
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1
-
-
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3
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-
-
-
-
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658163
Bogachev
Kinetics of the spectral chang ...
Vibrio harveyi
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2002
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392712
Steuber
Na+ translocation by bacterial ...
Escherichia coli, Klebsiella pneumoniae, Vibrio alginolyticus
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2001
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392713
Bogachev
: Sodium-dependent steps in th ...
Vibrio harveyi
Biochemistry
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7318-7323
2001
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FMN is covalently attached to ...
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Expression and mutagenesis of ...
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711995
Nakayama
Covalently bound flavin in the ...
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2000
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Nakayama
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392695
Pfenninger-Li
NADH:ubiquinone oxidoreductase ...
Vibrio alginolyticus
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1996
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Existence of Na+-translocating ...
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Cloning and sequencing of four ...
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392716
Tokuda
Na+ is translocated at NADH:qu ...
Vibrio alginolyticus
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7785-7790
1984
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