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Literature summary for 7.2.1.1 extracted from

  • Fadeeva, M.S.; Nunez, C.; Bertsova, Y.V.; Espin, G.; Bogachev, A.V.
    Catalytic properties of Na+-translocating NADH:quinone oxidoreductases from Vibrio harveyi, Klebsiella pneumoniae, and Azotobacter vinelandii (2008), FEMS Microbiol. Lett., 279, 116-123.
    View publication on PubMed
Search for more literature for 7.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-n-heptyl-4-hydroxyquinoline N-oxide inhibited by micromolar concentrations. In contrast to the corresponding enzymes from Vibrio harveyi and Klebsiella pneumoniae, the enzyme from Azotobacter vinelandii is resistant to low 2-n-heptyl-4-hydroxyquinoline N-oxide concentrations Azotobacter vinelandii
2-n-heptyl-4-hydroxyquinoline N-oxide
-
 Klebsiella pneumoniae
2-n-heptyl-4-hydroxyquinoline N-oxide submicromolar concentrations inhibit Vibrio harveyi
Ag+ 0.001 mM, partial and very slow inhibition Azotobacter vinelandii
Ag+
-
 Vibrio harveyi
diphenyliodonium modifies the noncovalently bound FAD of the enzyme Azotobacter vinelandii
diphenyliodonium modifies the noncovalently bound FAD of the enzyme Klebsiella pneumoniae
diphenyliodonium 0.05 mM, rapid inhibition, modifies the noncovalently bound FAD of the enzyme Vibrio harveyi
additional information the enzyme is fully resistant to either Ag+ or N-ethylmaleimide Azotobacter vinelandii
additional information the enzyme is fully resistant to either Ag+ or N-ethylmaleimide Klebsiella pneumoniae
NEM 5 mM, inhibits partially and very slowly Azotobacter vinelandii
NEM 5 mM, rapid inhibition Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km(Na+) is 0.0001 mM Azotobacter vinelandii
additional information
-
 additional information Km(Na+) is 0.067 mM Klebsiella pneumoniae
additional information
-
 additional information Km(Na+) is 0.67 mM Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae construction of a double NDH-2/NDH-1-deficient strain. Sub-bacterial particles isolated from these strains oxidize NADH entirely via the corresponding Na+-NQR, which makes them a useful tool to study the catalytic properties of these enzymes ?
-
 ?
additional information Vibrio harveyi construction of a NADH-dehydrogenase NDH-2-deficient strain. Sub-bacterial particles isolated from these strains oxidize NADH entirely via the corresponding Na+-NQR, which makes them a useful tool to study the catalytic properties of these enzymes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Klebsiella pneumoniae
-
-
-
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio harveyi
-
 Klebsiella pneumoniae
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information construction of a double NDH-2/NDH-1-deficient strain. Sub-bacterial particles isolated from these strains oxidize NADH entirely via the corresponding Na+-NQR, which makes them a useful tool to study the catalytic properties of these enzymes Klebsiella pneumoniae ?
-
 ?
additional information construction of a NADH-dehydrogenase NDH-2-deficient strain. Sub-bacterial particles isolated from these strains oxidize NADH entirely via the corresponding Na+-NQR, which makes them a useful tool to study the catalytic properties of these enzymes Vibrio harveyi ?
-
 ?
additional information Na+-translocating NADH:quinone oxidoreductases activity, the enzyme also shows NADH-oxidase activity Azotobacter vinelandii ?
-
 ?
additional information the enzyme also shows NADH-oxidase activity Vibrio harveyi ?
-
 ?
additional information the enzyme also shows NADH-oxidase activity Klebsiella pneumoniae ?
-
 ?

Synonyms

Synonyms Comment Organism
Na+-translocating NADH:quinone oxidoreductase
-
 Klebsiella pneumoniae
Na+-translocating NADH:quinone oxidoreductase
-
 Azotobacter vinelandii
Na+-translocatingNADH:quinone oxidoreductase
-
 Vibrio harveyi
Na1-NQR
-
 Vibrio harveyi
Na1-NQR
-
 Klebsiella pneumoniae
Na1-NQR
-
 Azotobacter vinelandii

Cofactor

Cofactor Comment Organism Structure
NADH no activity with NADPH Vibrio harveyi
NADH no activity with NADPH Klebsiella pneumoniae
NADH no activity with NADPH Azotobacter vinelandii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0001
-
-
 Vibrio harveyi Ag+
0.00013
-
-
 Vibrio harveyi 2-n-heptyl-4-hydroxyquinoline N-oxide
0.00055
-
-
 Klebsiella pneumoniae 2-n-heptyl-4-hydroxyquinoline N-oxide