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Literature summary for 7.2.1.1 extracted from
- Acid residues in the transmembrane helices of the Na+-pumping NADH:quinone oxidoreductase from vibrio cholerae involved in sodium translocation (2009), Biochemistry, 48, 9516-9524.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D133A | The mutants NqrB-E28A, NqrB-E144L, NqrD-D133A, and NqrD-D88L, in which sodium stimulation of sodium-dependent quinone reductase activity activity is lowered significantly but not eliminated, produces diminished levels of sodium pumping activity | Vibrio cholerae serotype O1 |
| D346A | NqrB-D397A. Sodium-dependent quinone reductase activity of the mutant enzyme is very similar to that of wild-type Na+-NQR in the absence of sodium. The mutant enzyme in which the sodium-dependent quinone reductase activity activity is insensitive to sodium is unable to form a sodium gradient. | Vibrio cholerae serotype O1 |
| D346A | the effect of sodium on sodium-dependent quinone reductase activity is almost eliminated. Little or no change in the KmappNa+ for sodium | Vibrio cholerae serotype O1 |
| D397A | sodium-dependent quinone reductase activity of mutant enzyme is very similar to that of wild-type Na+-NQR in the absence of sodium. The mutant enzyme in which the sodium-dependent quinone reductase activity is insensitive to sodium is unable to form a sodium gradient | Vibrio cholerae serotype O1 |
| D397A | the effect of sodium on sodium-dependent quinone reductase activity is almost eliminated | Vibrio cholerae serotype O1 |
| D88L | mutant enzyme shows little or no change in the KmappNa+ for sodium. The mutant enzyme in which sodium stimulation of sodium-dependent quinone reductase activity activity is lowered significantly but not eliminated, produces diminished levels of sodium pumping activity | Vibrio cholerae serotype O1 |
| E144L | NqrB-E144L. The mutant in which sodium stimulation of sodium-dependent quinone reductase activity is lowered significantly but not eliminated, produces diminished levels of sodium pumping activity | Vibrio cholerae serotype O1 |
| E28A | NqrB-D346A. The mutant in which sodium stimulation of sodium-dependent quinone reductase activity is lowered significantly but not eliminated, produces diminished levels of sodium pumping activity. Little or no change in the KmappNa+ for sodium | Vibrio cholerae serotype O1 |
| E95A | NqrE-E95A. The effect of sodium on sodium-dependent quinone reductase activity is almost eliminated. Sodium-dependent quinone reductase activity of mutant enzyme is very similar to that of wild-type Na+-NQR in the absence of sodium. The mutant enzyme in which the sodium-dependent quinone reductase activity activity is insensitive to sodium is unable to form a sodium gradient | Vibrio cholerae serotype O1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Kmapp(Na+) is: 1.1 mM for wild-type enzyme, 1.4 mM for subunit D mutant D88L, 9.3 mM for subunit D mutant D133A | Vibrio cholerae serotype O1 | |
| additional information | - |
additional information | Kmapp(Na+) is: 1.1 mM for wild-type enzyme, 13.1 mM for subunit E mutant E95A | Vibrio cholerae serotype O1 | |
| additional information | - |
additional information | Kmapp(Na+) is: 1.1 mM for wild-type enzyme, 2.1 mM for subunit B mutant E28A, 4.2 mM for subunit B mutant E144L, 0.9 mM for subunit B mutant D346A, above 100 mM for subunit B mutant D397A | Vibrio cholerae serotype O1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + ubiquinone | Vibrio cholerae serotype O1 | Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone | NAD+ + ubiquinol | - |
? |  |
| NADH + H+ + ubiquinone | Vibrio cholerae serotype O1 ATCC 39315 | Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone | NAD+ + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 | Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
| Vibrio cholerae serotype O1 ATCC 39315 | Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 | Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant enzyme E95A | Vibrio cholerae serotype O1 |
| mutant enzymes NqrB-D346A and NqrB-D397A | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + ubiquinone | Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone | Vibrio cholerae serotype O1 | NAD+ + ubiquinol | - |
? | |
| NADH + H+ + ubiquinone | Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone | Vibrio cholerae serotype O1 ATCC 39315 | NAD+ + ubiquinol | - |
? | |
| NADH + H+ + ubiquinone 1 | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol 1 | - |
? | |
| NADH + H+ + ubiquinone 1 | - |
Vibrio cholerae serotype O1 ATCC 39315 | NAD+ + ubiquinol 1 | - |
? | |
| NADH + H+ + ubiquinone-1 | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 | - |
? | |
| NADH + H+ + ubiquinone-1 | - |
Vibrio cholerae serotype O1 ATCC 39315 | NAD+ + ubiquinol-1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Na(+)-translocating NADH-quinone reductase subunit B | - |
Vibrio cholerae serotype O1 |
| Na(+)-translocating NADH-quinone reductase subunit D | - |
Vibrio cholerae serotype O1 |
| Na(+)-translocating NADH-quinone reductase subunit E | - |
Vibrio cholerae serotype O1 |
| Na+-NQR | - |
Vibrio cholerae serotype O1 |
| Na+-pumping NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Vibrio cholerae serotype O1 | |
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