Protein Variants | Comment | Organism |
---|---|---|
A553T | naturally occuring point mutation A553T plus deletion of residues from Leu554 to Ala558 in the transmembrane domain 12. The mutant fails to support heterotrophic growth after germination. Exogenous supplementation of succinate or the specific removal of the cytosolic diphosphate by the heterologous expression of the cytosolic inorganic pyrophosphatase 1, IPP1, gene from Saccharomyces cerevisiae rescues fugu5 phenotypes. Compared with the wild-type and AVP1Pro:IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant is severely compromised in the dark but recovers upon exogenous supply of succinate to the growth media. The peroxisomal beta-oxidation activity, dry seed contents of storage lipids, and their mobilization are unaffected in fugu5 | Arabidopsis thaliana |
A709T | the naturally occuring fugu5 mutant is defective in AVP1, i.e. vacuolar H+-pyrophosphatase, due to a point mutation A709T and fails to support heterotrophic growth after germination. Cell division is almost totally inhibited in cotyledons postembryonically, phenotype, overview, fails to support heterotrophic growth after germination. Exogenous supplementation of succinate or the specific removal of the cytosolic diphosphate by the heterologous expression of the cytosolic inorganic pyrophosphatase 1, IPP1, gene from Saccharomyces cerevisiae rescues fugu5 phenotypes. Compared with the wild-type and AVP1Pro:IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant is severely compromised in the dark but recovers upon exogenous supply of succinate to the growth media. The peroxisomal beta-oxidation activity, dry seed contents of storage lipids, and their mobilization are unaffected in fugu5 | Arabidopsis thaliana |
E272K | naturally occuring point mutation, fails to support heterotrophic growth after germination. Exogenous supplementation of succinate or the specific removal of the cytosolic diphosphate by the heterologous expression of the cytosolic inorganic pyrophosphatase 1, IPP1, gene from Saccharomyces cerevisiae rescues fugu5 phenotypes. Compared with the wild-type and AVP1Pro:IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant is severely compromised in the dark but recovers upon exogenous supply of succinate to the growth media. The peroxisomal beta-oxidation activity, dry seed contents of storage lipids, and their mobilization are unaffected in fugu5 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | vacuolar H+-pyrophosphatase | Arabidopsis thaliana | 5773 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Arabidopsis thaliana | - |
2 phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | A8MQH1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Arabidopsis thaliana | 2 phosphate + H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AVP1 | - |
Arabidopsis thaliana |
V-PPase | - |
Arabidopsis thaliana |
vacuolar H+-pyrophosphatase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the fugu5 mutant is defective in AVP1, i.e. vacuolar H+-pyrophosphatase, due to point mutations A709T, A553T, or E272K, and fails to support heterotrophic growth after germination. Exogenous supplementation of succinate or the specific removal of the cytosolic diphosphate by the heterologous expression of the cytosolic inorganic diphosphatase1, IPP1, gene from Saccharomyces cerevisiae rescues fugu5 phenotypes. Compared with the wild-type and AVP1Pro:IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant is severely compromised in the dark but recovers upon exogenous supply of succinate to the growth media. The peroxisomal beta-oxidation activity, dry seed contents of storage lipids, and their mobilization are unaffected in fugu5 | Arabidopsis thaliana |
physiological function | vacuolar H+-diphosphatase, AVP1, is a key enzyme in phosphate hydrolysis | Arabidopsis thaliana |