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Literature summary for 7.1.3.1 extracted from

  • Nakanishi, Y.; Saijo, T.; Wada, Y.; Maeshima, M.
    Mutagenic analysis of functional residues in putative substrate-binding site and acidic domains of vacuolar H+-pyrophosphatase (2001), J. Biol. Chem., 276, 7654-7660.
    View publication on PubMed
Search for more literature for 7.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
KCl 3.5fold increase at 50 mM Vigna radiata
additional information no activation with NaCl at 50 mM Vigna radiata

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae BJ5458 Vigna radiata

Protein Variants

Protein Variants Comment Organism
C304R complete loss of activity, no proton pumping Vigna radiata
D279A complete loss of activity, no proton pumping Vigna radiata
D279E complete loss of activity, no proton pumping Vigna radiata
D283A complete loss of activity, no proton pumping Vigna radiata
D283E complete loss of activity, no proton pumping Vigna radiata
D287A complete loss of activity, no proton pumping Vigna radiata
D287E complete loss of activity, no proton pumping Vigna radiata
D723A complete loss of activity, no proton pumping Vigna radiata
D723E complete loss of activity, no proton pumping Vigna radiata
D727A complete loss of activity, no proton pumping Vigna radiata
D727E complete loss of activity, no proton pumping Vigna radiata
D731A complete loss of activity, no proton pumping Vigna radiata
D731E complete loss of activity, no proton pumping Vigna radiata
E263D 50% of hydrolytic activity, no proton pumping Vigna radiata
E263G complete loss of activity, no proton pumping Vigna radiata
K261R 25% of hydrolytic activity, no proton pumping Vigna radiata
V259A 60% of hydrolytic activity, no proton pumping Vigna radiata

General Stability

General Stability Organism
Mg2+ partially stabilizes enzyme against tryptic digestion Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive to KF Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 Vigna radiata 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity and stability Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 SDS-PAGE Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Vigna radiata important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Vigna radiata 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane Vigna radiata 2 phosphate + H+[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
H+-translocating inorganic pyrophosphatase
-
 Vigna radiata