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Literature summary for 7.1.3.1 extracted from

  • Islam, M.K.; Miyoshi, T.; Kasuga-Aoki, H.; Isobe, T.; Arakawa, T.; Matsumoto, Y.; Tsuji, N.
    Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites (2003), Eur. J. Biochem., 270, 2814-2826.
    View publication on PubMed
Search for more literature for 7.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOP10F' Ascaris suum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Ascaris suum
Imidodiphosphate slight inhibition Ascaris suum
NaF
-
 Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.117
-
 diphosphate recombinant protein, pH 7.5, 55°C Ascaris suum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane type II enzyme, proton translocating form Ascaris suum 16020
-
soluble type I enzyme Ascaris suum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ascaris lumbricoides
Mg2+ required, highest activity at 5 mM, decrease of activity at higher concentrations Ascaris suum
Mg2+ required Toxocara canis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38770
-
 calculated from the predicted amino acid sequence after removal of the signal peptide Ascaris suum
39000
-
 SDS-PAGE Ascaris suum
40600
-
 calculated from predicted amino acid sequence of the entire ORF Ascaris suum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Ascaris suum important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] Ascaris lumbricoides important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] Toxocara canis important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Ascaris lumbricoides
-
-
-
Ascaris suum Q86M43 soluble enzyme
-
Toxocara canis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Ascaris suum

Source Tissue

Source Tissue Comment Organism Textmining
body wall
-
 Ascaris suum
-
gut epithelium
-
 Ascaris suum
-
ovary
-
 Ascaris suum
-
uterus
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
937
-
 recombinant protein, pH 7.5, 55°C Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Ascaris suum 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Ascaris lumbricoides 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Toxocara canis 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1]
-
 Ascaris lumbricoides 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1]
-
 Toxocara canis 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1]
-
 Ascaris suum 2 phosphate + H+[side 2]
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
638
-
 diphosphate recombinant protein, pH 7.5, 55°C Ascaris suum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Ascaris suum

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5
-
 Ascaris suum

pI Value

Organism Comment pI Value Maximum pI Value
Ascaris suum calculated from predicted amino acid sequence and detected in isoelectric focusing
-
 7.1