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Literature summary for 7.1.2.2 extracted from

  • Kawasaki-Nishi, S.; Nishi, T.; Forgac, M.
    Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation (2001), Proc. Natl. Acad. Sci. USA, 98, 12397-12402 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Protein Variants

Protein Variants Comment Organism
R735A the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R735C the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R735E the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R735K the mutant, although completely inactive for proton transport, retains 24% of wild type ATPase activity Saccharomyces cerevisiae
R735L the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R735N the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R735Q the mutant is fully assembled but is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R762A the mutant retains full wild type ATPase activity and about 90% of wild type proton transport activity Saccharomyces cerevisiae
R762K the mutant retains about 80% of wild type ATPase activity and about 80% of wild type proton transport activity Saccharomyces cerevisiae
R762L the mutant retains about 75% of wild type ATPase activity and about 85% of wild type proton transport activity Saccharomyces cerevisiae
R799A the mutant is almost totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae
R799K the mutant, which is almost completely inactive for proton transport, retains about 10% of wild type ATPase activity Saccharomyces cerevisiae
R799L the mutant is totally devoid of proton transport and ATPase activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Saccharomyces cerevisiae 5773
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+[side 1] Saccharomyces cerevisiae
-
 ADP + phosphate + H+[side 2]
-
 ?
ATP + H2O + H+[side 1] Saccharomyces cerevisiae MM112
-
 ADP + phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae MM112
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + H+[side 2]
-
 ?
ATP + H2O + H+[side 1]
-
 Saccharomyces cerevisiae MM112 ADP + phosphate + H+[side 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, subunit a, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
V-ATPase
-
 Saccharomyces cerevisiae
vacuolar (H+)-ATPase
-
 Saccharomyces cerevisiae
Vph1p subunit A Saccharomyces cerevisiae