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Literature summary for 7.1.2.2 extracted from

  • Ward, J.M.; Sze, H.
    Proton transport activity of the purified vacuolar H-ATPase from oats direct stimulation by Cl (1992), Plant Physiol., 99, 925-931 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
gramicidin D
-
Avena sativa
soybean phospholipid
-
Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
bafilomycin A1 completely inhibited by less than 50 nM bafilomycin A1 Avena sativa
additional information not inhibited by azide or vanadate Avena sativa
N,N'-dicyclohexylcarbodiimide
-
Avena sativa
NO3- 50 mM NO3- inhibits 90% of the hydrolytic activity, while H+ transport activity is decreased by 50% Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
Avena sativa 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- chloride stimulates both ATP hydrolysis by the purified ATPase and H+ pumping by the reconstituted ATPase in the presence of K+ and valinomycin Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
650000
-
gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+[side 1] Avena sativa
-
ADP + phosphate + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Superdex 200 gel filtration Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+[side 1]
-
Avena sativa ADP + phosphate + H+[side 2]
-
?

Subunits

Subunits Comment Organism
heterodecamer x * 70000 + x * 60000 + x * 44000 + x * 42000 + x * 36000 + x * 32000 + x * 29000 + x * 16000 + x * 13000 + x * 12000, SDS-PAGE Avena sativa

Synonyms

Synonyms Comment Organism
vacuolar H+-ATPase
-
Avena sativa
vacuolar H+-pumping ATPase
-
Avena sativa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000001
-
at pH and °C Avena sativa bafilomycin A1