Literature summary for 7.1.2.2 extracted from

Shirakihara, Y.; Shiratori, A.; Tanikawa, H.; Nakasako, M.; Yoshida, M.; Suzuki, T.
Structure of a thermophilic F1-ATPase inhibited by an epsilon-subunit: deeper insight into the epsilin-inhibition mechanism (2015), FEBS J., 282, 2895-2913.

Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged enzyme is expressed in FoF1-deficient Escherichia coli B834(DE)cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 14-16% (w/v) poly(ethyleneglycol) 6000, 0.2 M sodium chloride, 0.05 M Tris-sulfate buffer (pH 8.0), 5 mM CyDTA and 10% (v/v) ethylene glycol	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + H+/in	Escherichia coli	-	ADP + phosphate + H+/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0ABB0 and P0ABB4 and P0ABA6 and P0A6E6	alpha, beta, gamma, and epsilon subunits	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, DEAE Toyopearl column chromatography, and phenyl-Toyopearl column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Escherichia coli	ADP + phosphate + H+/out	-	?

Synonyms

Synonyms	Comment	Organism
F1-ATPase	catalytic sector in FoF1-ATP synthase	Escherichia coli

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Release 2023.1 (January 2023)