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Literature summary for 7.1.2.2 extracted from

  • Soga, N.; Kinosita, K.; Yoshida, M.; Suzuki, T.
    Efficient ATP synthesis by thermophilic Bacillus FoF1-ATP synthase (2011), FEBS J., 278, 2647-2654.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Protein Variants

Protein Variants Comment Organism
additional information generation of a mutant TFoF1 lacking an inhibitory segment of the epsilon-subunit, preparation of active proteoliposomes and for kinetic analysis of ATP synthesis, overview Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2-[[4-(trifluoromethoxy)phenyl]hydrazinylidene]propanedinitrile acts as an uncoupler and abolishes ATP synthesis Bacillus sp. (in: Bacteria)
nigericin acts as an uncoupler in the presence of valinomycin, and abolishes ATP synthesis Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, kinetic analysis of ATP synthesis, driven by acid-base transition and K+-diffusion potential, using active proteoliposomes Bacillus sp. (in: Bacteria)
0.013
-
 ADP pH not specified in the publication, 30°C Bacillus sp. (in: Bacteria)
0.55
-
 phosphate pH not specified in the publication, 30°C Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bacillus sp. (in: Bacteria)
-
 ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in Bacillus sp. (in: Bacteria) PS3
-
 ADP + phosphate + H+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) PS3
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information physiological growth temperature of Bacillus PS3 is 60°C or above Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Bacillus sp. (in: Bacteria) ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in FoF1-ATP synthase synthesizes ATP in the F1 portion when protons flow through Fo to rotate the shaft common to F1 and Fo, Kinetic analysis of ATP synthesis using active proteoliposomes Bacillus sp. (in: Bacteria) ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Bacillus sp. (in: Bacteria) PS3 ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in FoF1-ATP synthase synthesizes ATP in the F1 portion when protons flow through Fo to rotate the shaft common to F1 and Fo, Kinetic analysis of ATP synthesis using active proteoliposomes Bacillus sp. (in: Bacteria) PS3 ADP + phosphate + H+/out
-
 ?

Synonyms

Synonyms Comment Organism
FoF1
-
 Bacillus sp. (in: Bacteria)
FoF1-ATP synthase
-
 Bacillus sp. (in: Bacteria)
TFoF1
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 phosphate pH not specified in the publication, 30°C Bacillus sp. (in: Bacteria)
17
-
 ADP pH not specified in the publication, 30°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.65
-
 assay at, pH inside the liposomes Bacillus sp. (in: Bacteria)
8.8
-
 assay at, pH outside the liposomes Bacillus sp. (in: Bacteria)