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Literature summary for 7.1.2.2 extracted from

  • Mnatsakanyan, N.; Hook, J.A.; Quisenberry, L.; Weber, J.
    ATP synthase with its gamma subunit reduced to the N-terminal helix can still catalyze ATP synthesis (2009), J. Biol. Chem., 284, 26519-26525.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in enzyme-deficient Escherichia coli mutant strain DK8 Bacillus sp. PS3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacillus sp. PS3 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bacillus sp. PS3
-
 ADP + phosphate + H+/out
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant F1Fo-ATP synthase in membrane vesicles from enzyme-deficient Escherichia coli mutant strain DK8 Bacillus sp. PS3

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + 4 H+[side 1] = ADP + phosphate + 4 H+[side 2] ATP synthase uses a unique rotary mechanism to couple ATP synthesis and hydrolysis to transmembrane proton translocation. As part of the synthesis mechanism, the torque of the rotor has to be converted into conformational rearrangements of the catalytic binding sites on the stator to allow synthesis and release of ATP. The gamma subunit of the rotor plays a central role in the energy conversion. The N-terminal helix alone is able to fulfill the function of full-length gamma in ATP synthesis as long as it connects to the rest of the rotor. This connection can occur via the epsilon subunit. No direct contact between epsilon and the gamma ring seems to be required. The epsilon subunit of the rotor exists in two different conformations during ATP synthesis and ATP hydrolysis. Reaction mechanism, overview Bacillus sp. PS3
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Bacillus sp. PS3 ADP + phosphate + H+/out
-
 r

Synonyms

Synonyms Comment Organism
ATP synthase
-
 Bacillus sp. PS3
F1Fo
-
 Bacillus sp. PS3
F1FO-ATP synthase
-
 Bacillus sp. PS3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 assay at Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bacillus sp. PS3