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Literature summary for 7.1.2.2 extracted from

  • Enoki, S.; Watanabe, R.; Iino, R.; Noji, H.
    Single-molecule study on the temperature-sensitive reaction of F1-ATPase with a hybrid F1 carrying a single beta(E190D) (2009), J. Biol. Chem., 284, 23169-23176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Protein Variants

Protein Variants Comment Organism
alphaC193S/gammaS108C/I211C site-directed mutagenesis Bacillus sp. PS3
betaE190D site-directed mutagenesis, mutation of the beta subunit of F1 mutant alphaC193S/gammaS108C/I211C, the mutant shows a clear pause of the temperature-sensitive reaction below 18°C, the catalytic state of the temperature-sensitive reaction in rotation of the hybrid F1, carrying a single copy of betaE190D is observed at 18°C Bacillus sp. PS3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacillus sp. PS3 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bacillus sp. PS3
-
 ADP + phosphate + H+/out
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Bacillus sp. PS3 ADP + phosphate + H+/out
-
 r
ATP + H2O + H+/in the temperature-sensitive reaction is a structural rearrangement of beta subunit before or after ATP binding, overview Bacillus sp. PS3 ADP + phosphate + H+/out
-
 r

Synonyms

Synonyms Comment Organism
F1-ATPase
-
 Bacillus sp. PS3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Bacillus sp. PS3

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 highly temperature-sensitive reaction step of F1-ATPase from thermophilic Bacillus PS3 below 9°C as an intervening pause before the 80° substep at the same angle for ATP binding and ADP release. Temperature dependence of the rotation in wild-type and mutant enzymes, overview Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus sp. PS3

General Information

General Information Comment Organism
physiological function F1-ATPase is a rotary molecular motor in which the gamma-subunit rotates against the alpha3beta3 cylinder. The unitary gamma-rotation is a 120° step comprising 80° and 40° substeps, each of these initiated by ATP binding and ADP release and by ATP hydrolysis and inorganic phosphate release, respectively Bacillus sp. PS3