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Literature summary for 7.1.2.2 extracted from

  • Zanotti, F.; Gnoni, A.; Mangiullo, R.; Papa, S.
    Effect of the ATPase inhibitor protein IF1 on H+ translocation in the mitochondrial ATP synthase complex (2009), Biochem. Biophys. Res. Commun., 384, 43-48.
    View publication on PubMed
Search for more literature for 7.1.2.2

Inhibitors

Inhibitors Comment Organism Structure
42-58 IF1 synthetic peptide inhibits both H+ uptake and H+ release of the enzyme complex Bos taurus
IF1 protein the IF1 component of the mitochondrial complex is a basic 10 kDa protein, which inhibits the FoF1-ATP hydrolase activity, and both H+ uptake and H+ release. IF1, and in particular its central 42-58 segment, displays different inhibitory affinity for proton conduction from the F1 to the Fo side and in the opposite direction. Cross-linking of IF1 toF1-a/b subunits inhibits the ATP-driven H+ translocation but enhances H+ conduction in the reverse direction Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane the F1 moiety of the complex protrudes at the inner side of the membrane, the Fo sector spans the membrane reaching the outer side Bos taurus 5743
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bos taurus the H+ FoF1-ATP synthase complex of coupling membranes converts the proton-motive force into rotatory mechanical energy to drive ATP synthesis, the IF1 component of the mitochondrial complex is a basic 10 kDa protein, which inhibits the FoF1-ATP hydrolase activity ADP + phosphate + H+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native H+ FoF1-ATP synthase complex from bovine heart mitochondria by detergent solubilization, ultracentrifugation, and ultrafiltration Bos taurus

Renatured (Commentary)

Renatured (Comment) Organism
purified native H+ FoF1-ATP synthase complex after purification Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.05
-
 purified reconstituted enzyme complex, ATP hydrolysis, pH 8.0 Bos taurus
3.25
-
 purified reconstituted enzyme complex, ATP hydrolysis, pH 6.5 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Bos taurus ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in the H+ FoF1-ATP synthase complex of coupling membranes converts the proton-motive force into rotatory mechanical energy to drive ATP synthesis, the IF1 component of the mitochondrial complex is a basic 10 kDa protein, which inhibits the FoF1-ATP hydrolase activity Bos taurus ADP + phosphate + H+/out
-
 ?

Synonyms

Synonyms Comment Organism
H+ FoF1-ATP synthase
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0001
-
 inhibition of H+ uptake Bos taurus 42-58 IF1 synthetic peptide
0.00047
-
 inhibition of H+ uptake Bos taurus IF1 protein
0.00197
-
 inhibition of H+ release Bos taurus 42-58 IF1 synthetic peptide