Literature summary for 7.1.2.2 extracted from

De Vicente, J.I.; del Valle, P.; Busto, F.; de Attiaga, D.; Soler, J.
Mitochondrial F1-ATpase moiety from Phycomyces blakesleeanus: purification, characterization, and kinetic studies (1991), Biochem. Cell Biol., 69, 454-459.

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Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	negative allosteric effector	Phycomyces blakesleeanus
ATP	free ATP, MgATP2- is the true substrate	Phycomyces blakesleeanus
Mg2+	free Mg+ inhibits, MgATP is the true substrate	Phycomyces blakesleeanus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	ATP	-	Phycomyces blakesleeanus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	MgATP2- complex is the true substrate	Phycomyces blakesleeanus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus
25000	-	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus
31000	-	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus
53000	-	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus
60000	-	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus
390000	-	gel filtration	Phycomyces blakesleeanus

Organism

Organism	UniProt	Comment	Textmining
Phycomyces blakesleeanus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Phycomyces blakesleeanus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + 4 H+[side 1]	ATP in form of MgATP2-	Phycomyces blakesleeanus	ADP + phosphate + 4 H+[side 2]	-	?
ATP + H2O + H+/in	-	Phycomyces blakesleeanus	ADP + phosphate + H+/out	-	?

Subunits

Subunits	Comment	Organism
nonamer	alpha3,beta3,gamma1,delta1,epsilon1, 1 * 60000 + 3 * 53000 + 1 * 31000 + 1 * 25000 + 1 * 21000, SDS-PAGE	Phycomyces blakesleeanus

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Release 2023.1 (January 2023)