Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | neither octyl glucoside, 7-cyclohexyl-1-heptyl-beta-D-maltoside nor n-dodecyl-N,N-dimethylamine-N-oxide activate isoform Pma1p | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aluminum fluoride | - |
Saccharomyces cerevisiae | |
beryllium fluoride | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
ATP | activated enzyme state from plasma membrane, at pH 5.9 and 30°C | Saccharomyces cerevisiae | |
2.4 | - |
ATP | basal enzyme state from plasma membrane, at pH 5.9 and 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+[side 1] | Saccharomyces cerevisiae | - |
ADP + phosphate + H+[side 2] | - |
? | |
ATP + H2O + H+[side 1] | Saccharomyces cerevisiae YAK2 | - |
ADP + phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae YAK2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Reactive Red 120 bead chromatography | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
4°C, 50 mM MES/KOH (pH 6.5), 20% (v/v) glycerol, 50 mM KCl, 1 mM dithiothreitol, and 1 mM MgCl2, with and without inhibitors (1 mM aluminum fluoride or 1 mM beryllium fluoride), 3 weeks, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + H+[side 2] | - |
? | |
ATP + H2O + H+[side 1] | - |
Saccharomyces cerevisiae YAK2 | ADP + phosphate + H+[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Saccharomyces cerevisiae |
? | x * 996000, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
plasma membrane H+-ATPase | - |
Saccharomyces cerevisiae |
PM H+-ATPase | - |
Saccharomyces cerevisiae |
Pma1p | isoform | Saccharomyces cerevisiae |