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Literature summary for 7.1.2.1 extracted from

  • Pedersen, J.T.; Kanashova, T.; Dittmar, G.; Palmgren, M.
    Isolation of native plasma membrane H+-ATPase (Pma1p) in both the active and basal activation states (2018), FEBS Open Bio, 8, 774-783 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information neither octyl glucoside, 7-cyclohexyl-1-heptyl-beta-D-maltoside nor n-dodecyl-N,N-dimethylamine-N-oxide activate isoform Pma1p Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
aluminum fluoride
-
Saccharomyces cerevisiae
beryllium fluoride
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
ATP activated enzyme state from plasma membrane, at pH 5.9 and 30°C Saccharomyces cerevisiae
2.4
-
ATP basal enzyme state from plasma membrane, at pH 5.9 and 30°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Saccharomyces cerevisiae 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+[side 1] Saccharomyces cerevisiae
-
ADP + phosphate + H+[side 2]
-
?
ATP + H2O + H+[side 1] Saccharomyces cerevisiae YAK2
-
ADP + phosphate + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae YAK2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Reactive Red 120 bead chromatography Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
4°C, 50 mM MES/KOH (pH 6.5), 20% (v/v) glycerol, 50 mM KCl, 1 mM dithiothreitol, and 1 mM MgCl2, with and without inhibitors (1 mM aluminum fluoride or 1 mM beryllium fluoride), 3 weeks, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+[side 1]
-
Saccharomyces cerevisiae ADP + phosphate + H+[side 2]
-
?
ATP + H2O + H+[side 1]
-
Saccharomyces cerevisiae YAK2 ADP + phosphate + H+[side 2]
-
?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Saccharomyces cerevisiae
? x * 996000, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
plasma membrane H+-ATPase
-
Saccharomyces cerevisiae
PM H+-ATPase
-
Saccharomyces cerevisiae
Pma1p isoform Saccharomyces cerevisiae