Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.2.1 extracted from

  • Petrov, V.
    Role of loop L5-6 connecting transmembrane segments M5 and M6 in biogenesis and functioning of yeast Pma1 H+-ATPase (2015), Biochemistry, 80, 31-44.
    View publication on PubMed
Search for more literature for 7.1.2.1

Protein Variants

Protein Variants Comment Organism
D714A 5fold decrease in expression, loss of catalytic activity Saccharomyces cerevisiae
D714C mutation blocks trafficking, enzyme is not delivered to secretory vesicles. 4% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D714E 12% of wild-type expression, 7% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D714N 67% of wild-type expression, 97% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D718A 79% of wild-type expression, 104% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D718C 84% of wild-type expression, 108% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D720A 65% of wild-type expression, 47% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D720C 85% of wild-type expression, 80% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D720E 61% of wild-type expression, 87% of wild-type ATP hydrolysis Saccharomyces cerevisiae
D720N 72% of wild-type expression, 88% of wild-type ATP hydrolysis Saccharomyces cerevisiae
I719A 3fold reduction in coupling ratio between ATP hydrolysis and H+ transport Saccharomyces cerevisiae
I719C mutant is similar to wild-type Saccharomyces cerevisiae
L717A mutation blocks trafficking, enzyme is not delivered to secretory vesicles Saccharomyces cerevisiae
L717C mutation blocks trafficking, enzyme is not delivered to secretory vesicles Saccharomyces cerevisiae
N715A 68% of wild-type expression, 71% of wild-type ATP hydrolysis Saccharomyces cerevisiae
N715C 88% of wild-type expression, 58% of wild-type ATP hydrolysis Saccharomyces cerevisiae
S716A 71% of wild-type expression, 60% of wild-type ATP hydrolysis Saccharomyces cerevisiae
S716C 95% of wild-type expression, 101% of wild-type ATP hydrolysis Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
vanadate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 ATP mutant D720C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.5
-
 ATP mutant S716A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.6
-
 ATP mutant N715A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.7
-
 ATP mutant D720A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.7
-
 ATP mutant D720E, 30°C, pH 5.7 Saccharomyces cerevisiae
0.9
-
 ATP mutant D714N, 30°C, pH 5.7 Saccharomyces cerevisiae
0.9
-
 ATP mutant D720N, 30°C, pH 5.7 Saccharomyces cerevisiae
1
-
 ATP mutant I719A, 30°C, pH 5.7 Saccharomyces cerevisiae
1
-
 ATP mutant S716C, 30°C, pH 5.7 Saccharomyces cerevisiae
1.1
-
 ATP mutant D718A, 30°C, pH 5.7 Saccharomyces cerevisiae
1.2
-
 ATP mutant I719C, 30°C, pH 5.7 Saccharomyces cerevisiae
1.3
-
 ATP wild-type, 30°C, pH 5.7 Saccharomyces cerevisiae
1.6
-
 ATP mutant N715C, 30°C, pH 5.7 Saccharomyces cerevisiae
2.8
-
 ATP mutant D718C, 30°C, pH 5.7 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae SY4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae SY4 ADP + phosphate + H+/out
-
 ?

Synonyms

Synonyms Comment Organism
PMA1
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 vanadate mutant I719C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.001
-
 vanadate mutant N715C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.001
-
 vanadate mutant S716C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0011
-
 vanadate mutant D718C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0012
-
 vanadate mutant N715A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0012
-
 vanadate mutant S716A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0019
-
 vanadate mutant D720C, 30°C, pH 5.7 Saccharomyces cerevisiae
0.002
-
 vanadate mutant I719A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0027
-
 vanadate wild-type, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0033
-
 vanadate mutant D714N, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0035
-
 vanadate mutant D720E, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0037
-
 vanadate mutant D718A, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0037
-
 vanadate mutant D720N, 30°C, pH 5.7 Saccharomyces cerevisiae
0.0055
-
 vanadate mutant D720A, 30°C, pH 5.7 Saccharomyces cerevisiae