Literature summary for 7.1.2.1 extracted from

Buch-Pedersen, M.J.; Rudashevskaya, E.L.; Berner, T.S.; Venema, K.; Palmgren, M.G.
Potassium as an intrinsic uncoupler of the plasma membrane H+-ATPase (2006), J. Biol. Chem., 281, 38285-38292.

Search for more literature for 7.1.2.1

Protein Variants

Protein Variants	Comment	Organism
D617A	35% increase in catalytic activity	Arabidopsis thaliana
D617A/D684N	22% of wild-type activity, insensitive to vanadate	Arabidopsis thaliana
D684N	24% of wild-type activity, insensitve to vanadate	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
K+	K+ is an intrinsic uncoupler of the proton pump. Binding of K+ to the cytoplasmic phosphorylation domain can induce dephosphorylation of the phosphorylated E1P reaction cycle intermediate by a mechanism involving residue E184 in the conserved TGEs motif	Arabidopsis thaliana
vanadate	-	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	vanadate	mutant D617A, pH 6.5, 30°C	Arabidopsis thaliana
0.052	-	vanadate	mutant D617A/D684N, pH 6.5, 30°C	Arabidopsis thaliana
0.056	-	vanadate	mutant D684N, pH 6.5, 30°C	Arabidopsis thaliana
0.064	-	vanadate	wild-type, pH 6.5, 30°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Arabidopsis thaliana	ADP + phosphate + H+/out	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.4	-	mutants D684N, D617A/D684N	Arabidopsis thaliana
6.8	-	wild-type, mutant D217A	Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.01	-	vanadate	mutant D617A, pH 6.5, 30°C	Arabidopsis thaliana
0.01	-	vanadate	wild-type, pH 6.5, 30°C	Arabidopsis thaliana

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Release 2023.1 (January 2023)