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Literature summary for 7.1.2.1 extracted from

  • Sampedro, J.G.; Ruiz-Granados, Y.G.; Najera, H.; Tellez-Valencia, A.; Uribe, S.
    Fluorescence quenching by nucleotides of the plasma membrane H+-ATPase from Kluyveromyces lactis (2007), Biochemistry, 46, 5616-5622.
    View publication on PubMed
Search for more literature for 7.1.2.1

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-(beta,gamma-imido)-triphosphate 5 mM, 60% inhibition Kluyveromyces lactis
ADP 5 mM, 60% inhibition, Kd value 0.8 mM Kluyveromyces lactis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kluyveromyces lactis

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + 4 H+[side 1] = ADP + phosphate + 4 H+[side 2] binding of the nucleotide to the N-domain is coupled to the movement of a loop beta structure and to the exposure of the W505 residue located in the loop Kluyveromyces lactis
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in reaction is slightly cooperative, Hill number 1.5, S0.5 value 0.8 mM ATP. Kd value of ATP 0.7 mM Kluyveromyces lactis ADP + phosphate + H+/out
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 ATP pH 6.8, 25°C Kluyveromyces lactis