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Literature summary for 7.1.2.1 extracted from
- Purification and characterization of the proton translocating plasma membrane ATPase of red beet storage tissue (1990), Biochim. Biophys. Acta, 1039, 241-252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lysophosphatidylcholine | stimulates | Beta vulgaris |  |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| two-dimensional crystallization of the reconstituted enzyme | Beta vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dicyclohexylcarbodiimide | - |
Beta vulgaris | |
| Na2MO4 | weak | Beta vulgaris | |
| vanadate | Na3VO4, non-competitive | Beta vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
ATP | - |
Beta vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Beta vulgaris | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | KCl stimulates | Beta vulgaris | |
| Mg2+ | required | Beta vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
x * 100000, SDS-PAGE | Beta vulgaris |
| 600000 | - |
gel filtration | Beta vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains 80 mol of phospholipid per mol of 100000 Da ATPase, 60% phosphatidylcholine, 30% phosphatidylethanolamine, 5% lysophosphatidylcholine | Beta vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| storage tissue | - |
Beta vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.4 | - |
- |
Beta vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + 4 H+[side 1] | ATP in form of MgATP2- | Beta vulgaris | ADP + phosphate + 4 H+[side 2] | - |
? | |
| ATP + H2O + H+/in | - |
Beta vulgaris | ADP + phosphate + H+/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, SDS-PAGE | Beta vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Beta vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7 | about 45% of maximal activity at pH 5.4 and at pH 7.0 | Beta vulgaris |
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