Literature summary for 7.1.2.1 extracted from

Lanfermeijer, F.C.; Venema, K.; Palmgren, M.G.
Purification of histidine-tagged plant plasma membrane H+-ATPase expressed in yeast (1998), Protein Expr. Purif., 12, 29-37.

Search for more literature for 7.1.2.1

Inhibitors

Inhibitors	Comment	Organism	Structure
Na3VO4	0.1 mM	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	ATP	wild type enzyme and histidine-tagged enzyme	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Arabidopsis thaliana	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
96000	-	x * 96000, SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-affinity chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32.6	-	-	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Arabidopsis thaliana	ADP + phosphate + H+/out	-	?

Subunits

Subunits	Comment	Organism
?	x * 96000, SDS-PAGE	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
56.7	-	ATP	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.3	-	wild type enzyme	Arabidopsis thaliana
6.6	-	histidine-tagged enzyme	Arabidopsis thaliana

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Release 2023.1 (January 2023)