Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.9 extracted from

  • Kabashima, Y.; Ueda, N.; Sone, N.; Sakamoto, J.
    Mutation analysis of the interaction of B-type cytochrome c oxidase with its natural substrate cytochrome c-551 (2010), J. Biosci. Bioeng., 109, 325-330.
    View publication on PubMed
Search for more literature for 7.1.1.9

Protein Variants

Protein Variants Comment Organism
D49N the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
D99N the mutant shows strongly increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E116Q the mutant shows slightly reduced Km and strongly reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E139Q the mutant shows increased Km and slightly increased kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E64Q the mutant shows increased Km and kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E66Q the mutant shows increased Km and kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E68Q the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
E84Q the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000023
-
 reduced cytochrome c551 mutant enzyme E116Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000026
-
 reduced cytochrome c551 wild type enzyme, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000035
-
 reduced cytochrome c551 mutant enzyme E84Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000044
-
 reduced cytochrome c551 mutant enzyme D49N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000059
-
 reduced cytochrome c551 mutant enzyme E66Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000063
-
 reduced cytochrome c551 mutant enzyme E139Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000074
-
 reduced cytochrome c551 mutant enzyme E68Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.0001
-
 reduced cytochrome c551 mutant enzyme E64Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
0.000154
-
 reduced cytochrome c551 mutant enzyme D99N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Geobacillus thermodenitrificans 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5976
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans
17074
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans
60371
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
 formerly Bacillus stearothermophilus
-
Geobacillus thermodenitrificans K1041
-
 formerly Bacillus stearothermophilus
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced cytochrome c551 + O2 + H+
-
 Geobacillus thermodenitrificans oxidized cytochrome c551 + H2O
-
 ?
reduced cytochrome c551 + O2 + H+
-
 Geobacillus thermodenitrificans K1041 oxidized cytochrome c551 + H2O
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
cytochrome bo3 oxidase
-
 Geobacillus thermodenitrificans
cytochrome bo3-type cytochrome c oxidase
-
 Geobacillus thermodenitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
 reduced cytochrome c551 mutant enzyme E116Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
140
-
 reduced cytochrome c551 mutant enzyme D99N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
199
-
 reduced cytochrome c551 mutant enzyme E66Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
200
-
 reduced cytochrome c551 mutant enzyme D49N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
290
-
 reduced cytochrome c551 mutant enzyme E68Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
295
-
 reduced cytochrome c551 wild type enzyme, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
297
-
 reduced cytochrome c551 mutant enzyme E139Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
317
-
 reduced cytochrome c551 mutant enzyme E64Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
325
-
 reduced cytochrome c551 mutant enzyme E84Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
heme
-
 Geobacillus thermodenitrificans