Cloned (Comment) | Organism |
---|---|
Production of cytochrome c oxidase deficient strains and ANR deficient strain. | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | 1 mM inhibits all terminal oxidases excepting the CIO quinol oxidase and lead to reduced NADH oxidation between 2.5- and 4fold | Pseudomonas putida | |
additional information | Inactivation of the Aa3 oxidase lead to a 50% reduction in N,N,N',N'-tetramethyl-1,4-benzenediamine (TMPD)-dependent oxidase activity (electron donor specific for cytochrome c-dependent oxidases). Lack of the Cbb3-2 oxidase lead to 65% reduction.; In cells growing exponentially under high oxygen tension (100% air saturation), the absence of ANR lead to a 20fold decrease in mRNA levels of the Cbb3-1 oxidase gene, a decrease that is 10-fold lower than that observed in cells growing exponentially in shaken flasks, and 35-fold lower than in cells entering the stationary phase. When cells are grown under limiting oxygen supply (40% air saturation), the absence of ANR lead to a over 500fold decrease in the mRNA levels of the Cbb3-1 oxidase gene; Under aerobic conditions, inactivation of transcriptional activator gene ANR lead to a significant decrease (more than 230fold) of the mRNA corresponding to the Cbb3-1 oxidase, but have little effect on the other analysed terminal oxidases Cbb3-2 and Aa3. | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas putida | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | For all mutant strains, the NADH oxidation activity of membranes obtained from aerobic exponentially growing cultures is some higher than that of the wild-type strain (in the range of 1035% higher) | ? | - |
? | |
additional information | Pseudomonas putida | inactivation of the Cbb3-1 terminal oxidase in a strain containing a wild-type ANR gene lead to a 46% decrease in cytochrome b and to a 57% decrease in cytochrome c | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | For all mutant strains, the NADH oxidation activity of membranes obtained from aerobic exponentially growing cultures is some higher than that of the wild-type strain (in the range of 1035% higher) | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | inactivation of the Cbb3-1 terminal oxidase in a strain containing a wild-type ANR gene lead to a 46% decrease in cytochrome b and to a 57% decrease in cytochrome c | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88RM6 | Accession number of subunit 2 of Aa3 oxidase | - |
Pseudomonas putida KT 2240 | Q88RM6 | Accession number of subunit 2 of Aa3 oxidase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | For all mutant strains, the NADH oxidation activity of membranes obtained from aerobic exponentially growing cultures is some higher than that of the wild-type strain (in the range of 1035% higher) | Pseudomonas putida | ? | - |
? | |
additional information | inactivation of the Cbb3-1 terminal oxidase in a strain containing a wild-type ANR gene lead to a 46% decrease in cytochrome b and to a 57% decrease in cytochrome c | Pseudomonas putida | ? | - |
? | |
additional information | For all mutant strains, the NADH oxidation activity of membranes obtained from aerobic exponentially growing cultures is some higher than that of the wild-type strain (in the range of 1035% higher) | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | inactivation of the Cbb3-1 terminal oxidase in a strain containing a wild-type ANR gene lead to a 46% decrease in cytochrome b and to a 57% decrease in cytochrome c | Pseudomonas putida KT 2240 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aa3 terminal Oxidase | - |
Pseudomonas putida |
cbb3-2 oxidase | - |
Pseudomonas putida |
cbb3-2 terminal oxidase | - |
Pseudomonas putida |
cytochrome aa3 | - |
Pseudomonas putida |