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Literature summary for 7.1.1.9 extracted from

  • Urbani, A.; Gemeinhardt, S.; Warne, A.; Saraste, M.
    Properties of the detergent solubilised cytochrome c oxidase (cytochrome cbb(3)) purified from Pseudomonas stutzeri (2001), FEBS Lett., 508, 29-35.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion, 200 mM Zn acetate, 100 mM Na acetate, pH 4.5, 19-22% polyethylene glycol 1000, 10 mg/ml enzyme concentration, hexagonal red crystals appear after 3 d at 20°C Pseudomonas stutzeri

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas stutzeri 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu 0.96 mol Cu per mol of enzyme Pseudomonas stutzeri
Fe 5.3 Fe atoms per enzyme complex Pseudomonas stutzeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23470
-
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry Pseudomonas stutzeri
26000
-
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE Pseudomonas stutzeri
34840
-
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry Pseudomonas stutzeri
35000
-
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE Pseudomonas stutzeri
41000
-
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE Pseudomonas stutzeri
58360
-
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry Pseudomonas stutzeri
114000
-
analytical ultracentrifugation Pseudomonas stutzeri
190000
-
gel filtration Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl, Cu2+-immobilized affinity chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + O2 artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine Pseudomonas stutzeri ferricytochrome c + H2O
-
?
N,N,N',N'-tetramethyl-4-phenylendiamine + O2 + H+
-
Pseudomonas stutzeri ?
-
?

Subunits

Subunits Comment Organism
trimer 1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry Pseudomonas stutzeri
trimer 1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
180
-
N,N,N',N'-tetramethyl-4-phenylendiamine
-
Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
heme b non-covalently bound Pseudomonas stutzeri
heme c non-covalently bound Pseudomonas stutzeri