Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.9 extracted from

  • Zhen, Y.; Hoganson, C.W.; Babcock, G.T.; Ferguson-Miller, S.
    Definition of the interaction domain for cytochrome c on cytochrome c oxidase. I. Biochemical, spectral, and kinetic characterization of surface mutants in subunit ii of Rhodobacter sphaeroides cytochrome aa(3) (1999), J. Biol. Chem., 274, 38032-38041.
    View publication on PubMed
Search for more literature for 7.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type, E148Q, D195N, E157Q, D214N, D188Q/E189N, D151Q/E152N, W143F, W143A and Y144A/W145A in Rhodobacter sphaeroides Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
D151Q/E152N similar steady-state kinetic as wild-type Cereibacter sphaeroides
D188Q/E189N similar steady-state kinetic as wild-type Cereibacter sphaeroides
D195N decrease in activity, increase in Km Cereibacter sphaeroides
D214N decrease in activity, increase in Km Cereibacter sphaeroides
E148Q decrease in activity, increase in Km Cereibacter sphaeroides
E157Q decrease in activity, increase in Km Cereibacter sphaeroides
W143A similar copper/Fe ratios as wild-type Cereibacter sphaeroides
W143F similar copper/Fe ratios as wild-type Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information comparison of high and low affinity Km values at low ionic strength with Rhodobacter spaeroides and horse cytochrome c of wild-type and various mutants Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + O2 + H+
-
 Cereibacter sphaeroides ferricytochrome c + H2O
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information highest turnover number at 55 mM ionic strength: D214N, D195N and E148Q, highest turnover number at 45 mM ionic strength: E157Q, comparison of high and low affinity turnover numbers at low ionic strength with Rhodobacter spaeroides and horse cytochrome c of wild-type and various mutants Cereibacter sphaeroides
6
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, W143A mutant enzyme Cereibacter sphaeroides
20
-
 ferrocytochrome c horse cytochrome c, W143A mutant enzyme Cereibacter sphaeroides
20
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, W143F mutant enzyme Cereibacter sphaeroides
40
-
 ferrocytochrome c horse cytochrome c, W143F mutant enzyme Cereibacter sphaeroides
50
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D229N mutant enzyme Cereibacter sphaeroides
54
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D195Q mutant enzyme Cereibacter sphaeroides
100
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, E157Q and E254A mutant enzyme Cereibacter sphaeroides
180
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D214N mutant enzyme Cereibacter sphaeroides
220
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D151N/E152Q mutant enzyme Cereibacter sphaeroides
230
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, E148Q mutant enzyme Cereibacter sphaeroides
260
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D188N/E189Q mutant enzyme Cereibacter sphaeroides
270
-
 ferrocytochrome c Rhodobacter sphaeroides cytochrome c, wild-type enzyme Cereibacter sphaeroides
290
-
 ferrocytochrome c wild-type enzyme, horse cytochrome c, 155 mM ionic strength Cereibacter sphaeroides
400
-
 ferrocytochrome c horse cytochrome c, D214N and D229N mutant enzyme Cereibacter sphaeroides
590
-
 ferrocytochrome c wild-type enzyme, horse cytochrome c, 5 mM ionic strength Cereibacter sphaeroides
600
-
 ferrocytochrome c horse cytochrome c, E157Q and E254Amutant enzyme Cereibacter sphaeroides
980
-
 ferrocytochrome c wild-type, D188N/E189Q and D151N/E152Q mutant enzyme, horse cytochrome c, 75 mM ionic strength Cereibacter sphaeroides
1100
-
 ferrocytochrome c horse cytochrome c, E148Q mutant enzyme Cereibacter sphaeroides
1200
-
 ferrocytochrome c horse cytochrome c, D195Q mutant enzyme Cereibacter sphaeroides
1300
-
 ferrocytochrome c horse cytochrome c, D151N/E152Q mutant enzyme Cereibacter sphaeroides
1600
-
 ferrocytochrome c horse cytochrome c, D188N/E189Q mutant enzyme Cereibacter sphaeroides
1700
-
 ferrocytochrome c horse cytochrome c, wild-type enzyme Cereibacter sphaeroides