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Literature summary for 7.1.1.9 extracted from
- Cytochrome a-type terminal oxidase derived from Thiobacillus novellus. Molecular and enzymatic properties (1980), Biochim. Biophys. Acta, 591, 53-62.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | 0.014 mM, 50% inhibition of tuna cytochrome c oxidation | Starkeya novella |  |
| CN- | 0.004 mM, 50% inhibition of tuna cytochrome c oxidation | Starkeya novella | |
| phosphate | not with yeast cytochrome c | Starkeya novella | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
ferrocytochrome c | - |
Starkeya novella | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
| 23000 | - |
x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
| 32000 | - |
1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
| 32000 | - |
x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Starkeya novella | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Triton X-100, ammonium sulfate, DEAE-cellulose, Sephadex G-150 | Starkeya novella |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
- |
Starkeya novella |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c + O2 | Saccharomyces cerevisiae cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | horse ferrocytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | cow cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | tuna cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 + H+ | - |
Starkeya novella | ferricytochrome c + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
| dimer | 1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | above 20 mM phosphate: rapid decrease of kcat with Candida krusei, tuna, Thiobacillus novellus, and horse cytochrome c | Starkeya novella | |
| 20 | - |
ferrocytochrome c | Thiobacillus novellus cytochrome c, 12.5 mM phosphate at pH 5.5 and 20°C | Starkeya novella | |
| 45.8 | - |
ferrocytochrome c | Candida krusei cytochrome c, 30 mM phosphate at pH 5.5 and 20°C | Starkeya novella | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
phosphate buffer | Starkeya novella |
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