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Literature summary for 7.1.1.9 extracted from
- A cytochrome aa3-type terminal oxidase of a thermophilic bacterium. Purification, properties and proton pumping (1982), Biochim. Biophys. Acta, 682, 216-226.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipid | 10fold activation | Bacillus sp. PS3 |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Bacillus sp. PS3 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
ferrocytochrome c | - |
Bacillus sp. PS3 | |
| 0.067 | - |
ferrocytochrome c | Saccharomyces cerevisiae cytochrome c | Bacillus sp. PS3 | |
| 0.13 | - |
ferrocytochrome c | hose cytochrome c | Bacillus sp. PS3 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | - |
Bacillus sp. PS3 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 56000 + 1 * 38000 + 1 * 22000, SDS-PAGE, Ferguson plot | Bacillus sp. PS3 |
| 38000 | - |
1 * 56000 + 1 * 38000 + 1 * 22000, SDS-PAGE, Ferguson plot | Bacillus sp. PS3 |
| 56000 | - |
1 * 56000 + 1 * 38000 + 1 * 22000, SDS-PAGE, Ferguson plot | Bacillus sp. PS3 |
| 250000 | - |
gel filtration in the presence of N-lauryl sarcosinate | Bacillus sp. PS3 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. PS3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Triton X-100, DEAE-cellulose, DEAE-toyopearl, hydroxyapatite, ammonium sulfate | Bacillus sp. PS3 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bacillus sp. PS3 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c + O2 | Saccharomyces cerevisiae cytochrome c | Bacillus sp. PS3 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | artificial electron donor: 2,6-dichlorophenolindophenol | Bacillus sp. PS3 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | artificial electron donor: phenazine methosulfate | Bacillus sp. PS3 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Bacillus sp. PS3 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 + H+ | - |
Bacillus sp. PS3 | ferricytochrome c + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 56000 + 1 * 38000 + 1 * 22000, SDS-PAGE, Ferguson plot | Bacillus sp. PS3 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
two-third of optimal activity at pH 5.0 and pH 7.1 | Bacillus sp. PS3 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.1 | less than 75% of maximal activity above and below | Bacillus sp. PS3 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme a | - |
Bacillus sp. PS3 | |
| heme c | - |
Bacillus sp. PS3 | |
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