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Literature summary for 7.1.1.9 extracted from

  • Artzabatov, V.; Muller, M.; Azzi, A.
    Isolation and partial characterization of the cytochrome c oxidase of Micrococcus luteus (lysodeikticus) (1987), Arch. Biochem. Biophys., 257, 476-480.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 47000 + x * 31000 + x * 19000, SDS-PAGE Micrococcus luteus
31000
-
x * 47000 + x * 31000 + x * 19000, SDS-PAGE Micrococcus luteus
47000
-
x * 47000 + x * 31000 + x * 19000, SDS-PAGE Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
lysodeikticus
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, ammonium sulfate, ion-exchange, affinity chromatography on yeast cytochrome c-Sepharose Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diaminodurene + O2 + H+
-
Micrococcus luteus ?
-
?
ferrocytochrome c + O2 artificial electron donor: ascorbate/diaminodurene Micrococcus luteus ferricytochrome c + H2O
-
?
ferrocytochrome c + O2 artificial electron donor: phenazine methosulfate Micrococcus luteus ferricytochrome c + H2O
-
?
ferrocytochrome c + O2 artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine Micrococcus luteus ferricytochrome c + H2O
-
?
N,N,N',N'-tetramethyl-4-phenylendiamine + O2 + H+
-
Micrococcus luteus ?
-
?
phenazine methosulfate + O2 + H+
-
Micrococcus luteus ?
-
?

Subunits

Subunits Comment Organism
? x * 47000 + x * 31000 + x * 19000, SDS-PAGE Micrococcus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.93
-
N,N,N',N'-tetramethyl-4-phenylendiamine ascorbate/N,N,N',N'-tetramethyl-p-phenylendiamine assay system Micrococcus luteus
15.3
-
diaminodurene ascorbate/diaminodurene assay system Micrococcus luteus
24.3
-
phenazine methosulfate ascorbate/phenazine methosulfate assay system Micrococcus luteus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00035
-
CN-
-
Micrococcus luteus