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Literature summary for 7.1.1.8 extracted from
- Nonoxidizable ubiquinol derivatives that are suitable for the study of the ubiquinol oxidation site in the cytochrome bc1 complex (2002), Biochim. Biophys. Acta, 1556, 226-232.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified cytochrome bc1 complex with bound inhibitors 2,3,4-trimethoxy-5-decyl-6-methyl-phenol or 2,3,4-trimethoxy-5-methyl-6-decyl-phenol, the latter in a 4fold molar excess, 2-4 weeks, X-ray diffraction structure determination and analysis | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3,4-trimethoxy-5-decyl-6-methyl-phenol | competitive inhibitor, binds stably to the Qo site of the cytochrome bc1 complex, binding structure, inhibitory potency depends on the chain length of the ubiquinol substrate and is higher with Q0C5 than with Q0C10, inhibition is pH-dependent | Bos taurus |  |
| 2,3,4-trimethoxy-5-methyl-6-decyl-phenol | competitive inhibitor, binds stably to the Qo site of the cytochrome bc1 complex, binding structure, inhibition is pH-dependent | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
ubiquinol | pH 7.4, 25°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquinol-2 + ferricytochrome c | Bos taurus | the cytochrome bc1 complex is the central segment of the respiratory chain in mitochondria | ubiquinone-2 + ferrocytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
enzyme is part of the cytochrome bc1 complex | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+[side 2] | substrate binding mechanism at the Qo site of the cytochrome bc1 complex | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquinol-2 + ferricytochrome c | the cytochrome bc1 complex is the central segment of the respiratory chain in mitochondria | Bos taurus | ubiquinone-2 + ferrocytochrome c | - |
? | |
| ubiquinol-2 + ferricytochrome c | substrate of chain length C5 to C10, ubiquinol binds transiently at the Qo site, only when both heme bL and the iron sulfur cluster are in the oxidized form, where it is oxidized | Bos taurus | ubiquinone-2 + ferrocytochrome c | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme is part of the mitochondrial cytochrome bc1 complex | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at, cytochrome bc1 complex | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
cytochrome bc1 complex | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | cytochrome bc1 complex | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0068 | - |
2,3,4-trimethoxy-5-decyl-6-methyl-phenol | pH 7.4, 25°C | Bos taurus | |
| 0.0716 | - |
2,3,4-trimethoxy-5-methyl-6-decyl-phenol | pH 7.4, 25°C | Bos taurus | |
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Release 2023.1 (January 2023)
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