Crystallization (Comment) | Organism |
---|---|
crystallization of the enzyme cytochrome bc1 complex with mitochondrial cytochrome c and the antibody fragment FV18E11, protein solution with 120 mM ionic strength is rapidly mixed with precipitation solution containing 12% PEG 4000, 20 mM Tris-HCl, pH 7.5, hanging drop vapour diffusion method, 3 weeks at 4°C, X-ray diffraction structure determination and analysis, modeling | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral | Saccharomyces cerevisiae | 16020 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme complex contains a [2Fe-2S] cluster | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-2 + 2 ferricytochrome c | Saccharomyces cerevisiae | electron transfer between yeast cytochrome bc1 complex and cytochrome c is coupled to proton transport across the inner mitochondrial membrane delivering a membrane potential, enzyme complex is important in cell respiration and photosynthesis | ubiquinone-2 + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+[side 2] | binding structure and mode of cytochrome c1 within the enzyme complex and cytochrome c during the catalytic reaction, direct heme-to-heme electron transfer from the enzyme complex cytochrome c1 to cytochrome c, half-of-the sites mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-2 + 2 ferricytochrome c | electron transfer between yeast cytochrome bc1 complex and cytochrome c is coupled to proton transport across the inner mitochondrial membrane delivering a membrane potential, enzyme complex is important in cell respiration and photosynthesis | Saccharomyces cerevisiae | ubiquinone-2 + 2 ferrocytochrome c + 2 H+ | - |
? | |
ubiquinol-2 + 2 ferricytochrome c | activity is highest near physiological ionic strength and decreases at higher concentrations | Saccharomyces cerevisiae | ubiquinone-2 + 2 ferrocytochrome c + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | complex composition, binding structure of cytochromes, crystal structure, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
cytochrome bc1 complex | - |
Saccharomyces cerevisiae |
QCR | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rate of direct heme-to-heme electron transfer from the enzyme complex cytochrome c1 to cytochrome c | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |