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Literature summary for 7.1.1.8 extracted from
- Characterization and resolution of complex III from beef heart mitochondria (1978), Methods Enzymol., 53, 80-91.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Antimycin | - |
Bos taurus |  |
| Antimycin | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE | Bos taurus |
| 13000 | - |
1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE | Bos taurus |
| 15000 | - |
1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE | Bos taurus |
| 26000 | - |
iron-sulfur protein, SDS-PAGE | Bos taurus |
| 29000 | - |
cytochrome c1, SDS-PAGE | Bos taurus |
| 47000 | - |
core protein 2, SDS-PAGE | Bos taurus |
| 50000 | - |
core protein 1, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, isolated complex III is stored in presence of 50% glycerol, can be used for functional studies up to a month, for preparation of subunits only freshly prepared bc1 complex, because storage leads to partial degradation of some subunits by proteases | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetramethyl-p-benzoquinol + cytochrome c | duroquinol | Bos taurus | tetramethyl-p-benzoquinone + reduced cytochrome c | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Bos taurus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heptamer | - |
Saccharomyces cerevisiae |
| heptamer | 1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b-562 | - |
Bos taurus | |
| cytochrome b-565 | - |
Bos taurus |
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