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Literature summary for 7.1.1.5 extracted from

  • Gartner, P.
    Characterization of a quinole-oxidase activity in crude extracts of Thermoplasma acidophilum and isolation of an 18-kDa cytochrome (1991), Eur. J. Biochem., 200, 215-222 .
    View publication on PubMed
Search for more literature for 7.1.1.5

General Stability

General Stability Organism
addition of total lipids from Thermoplasma acidophilum increases stability Thermoplasma acidophilum
asolectin from soya beans decreases stability Thermoplasma acidophilum
stability is lowered by the presence of detergent Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-3-undecyl-1,4-naphthoquinone 0.01 mM, 20% inhibition Thermoplasma acidophilum
2n-heptyl-4-hydroxyquinoline N-oxide most potent inhibitor Thermoplasma acidophilum
asolectin
-
 Thermoplasma acidophilum
Lipid addition of total lipids from Thermoplasma acidophilum decreases activity Thermoplasma acidophilum
additional information antimycin A, myxothiazol, methyl 3-methoxy-2-(2-styrylphenyl) propenate and stigmatellin (at concentration up to 0.030 mM) and Zn2+ at a concentration up to 1 mM, do not inhibit Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 ubiquinol-10 pH 5.4, 50°C Thermoplasma acidophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Thermoplasma acidophilum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thermoplasmaquinol + O2 + H+[side 1] Thermoplasma acidophilum likely candidate to the physiological electron donor thermoplasmaquinone + H2O + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dimethylnaphthohydroquinone + O2 + H+[side 1]
-
 Thermoplasma acidophilum ?
-
 ?
menadione + O2 + H+[side 1]
-
 Thermoplasma acidophilum ?
-
 ?
menaquinol-4 + O2 + H+[side 1] menaquinols are oxidized at 6-12fold higher rates than ubiquinols Thermoplasma acidophilum menaquinone-4 + H2O + H+[side 2]
-
 ?
thermoplasmaquinol + O2 + H+[side 1] likely candidate to the physiological electron donor Thermoplasma acidophilum thermoplasmaquinone + H2O + H+[side 2]
-
 ?
ubiquinol-1 + O2 + H+[side 1]
-
 Thermoplasma acidophilum ubiquinone-1 + H2O + H+[side 2]
-
 ?
ubiquinol-10 + O2 + H+[side 1] menaquinols are oxidized at 6-12fold higher rates than ubiquinols. However, because of the greater tendency for autoxidation of menaquinols, ubiquinol is a more convenient substrate for the enzyme assay Thermoplasma acidophilum ubiquinone-10 + H2O + H+[side 2]
-
 ?
ubiquinol-6 + O2 + H+[side 1]
-
 Thermoplasma acidophilum ubiquinone-6 + H2O + H+[side 2]
-
 ?

Subunits

Subunits Comment Organism
oligomer 12-16 * 14000, SDS-PAGE Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
Q10H2 oxidase
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
cytochrome cytochrome-o-type quinol oxidase Thermoplasma acidophilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
 2n-heptyl-4-hydroxyquinoline N-oxide pH 5.4, 50°C Thermoplasma acidophilum