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Literature summary for 7.1.1.5 extracted from
- Plasticity in the high affinity menaquinone binding site of the cytochrome aa3-600 menaquinol oxidase from Bacillus subtilis (2015), Biochemistry, 54, 5030-5044 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL10-Gold cells | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R70H | the mutant shows reduced activity compared to the wild type enzyme | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
dimethylnitrosamine | mutant enzyme R70H, at pH 6.8 and 25°C | Bacillus subtilis |  |
| 0.089 | - |
dimethylnitrosamine | wild type enzyme, at pH 6.8 and 25°C | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| menaquinol + O2 | Bacillus subtilis | - |
menaquinone + H2O | - |
? | |
| menaquinol + O2 | Bacillus subtilis 1A1 | - |
menaquinone + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 1A1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylnitrosamine + O2 | - |
Bacillus subtilis | ? | - |
? | |
| dimethylnitrosamine + O2 | - |
Bacillus subtilis 1A1 | ? | - |
? | |
| menaquinol + O2 | - |
Bacillus subtilis | menaquinone + H2O | - |
? | |
| menaquinol + O2 | - |
Bacillus subtilis 1A1 | menaquinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyt aa3-600 | - |
Bacillus subtilis |
| cytochrome aa3-600 menaquinol oxidase | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 180 | - |
dimethylnitrosamine | wild type enzyme, at pH 6.8 and 25°C | Bacillus subtilis | |
| 227 | - |
dimethylnitrosamine | mutant enzyme R70H, at pH 6.8 and 25°C | Bacillus subtilis | |
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Release 2023.1 (January 2023)
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