BRENDA - Enzyme Database
show all sequences of 7.1.1.4

Cytochrome aa3 from Sulfolobus acidocaldarius. A single-subunit, quinol-oxidizing archaebacterial terminal oxidase

Anemuller, S.; Schäfer, G.; Eur. J. Biochem. 191, 297-305 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
azide
20 mM, complete inhibition
Sulfolobus acidocaldarius
Cl-
70 mM, 50% inhibition
Sulfolobus acidocaldarius
cyanide
1-3 mM, complete inhibition
Sulfolobus acidocaldarius
phosphate
23 mM, 50% inhibition
Sulfolobus acidocaldarius
SO42-
28 mM, 50% inhibition
Sulfolobus acidocaldarius
Sulfide
1-3 mM, complete inhibition
Sulfolobus acidocaldarius
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride
pH 7.0, 40°C
Sulfolobus acidocaldarius
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
the single subunit (38000-40000 Da) contains two copper ions
Sulfolobus acidocaldarius
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000
Sulfolobus acidocaldarius
100000
104000
gel filtration
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
caldariellaquinol + O2 + n H+/in
Sulfolobus acidocaldarius
-
caldariellaquinone + H2O + n H+/out
-
-
?
caldariellaquinol + O2 + n H+/in
Sulfolobus acidocaldarius DSM 639
-
caldariellaquinone + H2O + n H+/out
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius DSM 639
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36
-
pH 7.0, 40°C, oxidation of N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride
Sulfolobus acidocaldarius
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
caldariella quinol + O2 + n H+/in
isolated aa3 preparation does not oxidize cytochrome c
396010
Sulfolobus acidocaldarius
caldariella quinone + H2O + n H+/out
-
-
-
?
caldariella quinol + O2 + n H+/in
isolated aa3 preparation does not oxidize cytochrome c
396010
Sulfolobus acidocaldarius DSM 639
caldariella quinone + H2O + n H+/out
-
-
-
?
caldariellaquinol + O2 + n H+/in
-
396010
Sulfolobus acidocaldarius
caldariellaquinone + H2O + n H+/out
-
-
-
?
caldariellaquinol + O2 + n H+/in
-
396010
Sulfolobus acidocaldarius DSM 639
caldariellaquinone + H2O + n H+/out
-
-
-
?
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
396010
Sulfolobus acidocaldarius
?
-
-
-
?
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
396010
Sulfolobus acidocaldarius DSM 639
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38000
Sulfolobus acidocaldarius
Cofactor
Cofactor
Commentary
Organism
Structure
heme
hemeprotein. The single subunit (38000-40000 Da) contains two heme a molecules. The redox potentials of the heme centres are +220 mV and +370 mV, respectively. One heme is a heme-a3 centre
Sulfolobus acidocaldarius
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
hemeprotein. The single subunit (38000-40000 Da) contains two heme a molecules. The redox potentials of the heme centres are +220 mV and +370 mV, respectively. One heme is a heme-a3 centre
Sulfolobus acidocaldarius
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
azide
20 mM, complete inhibition
Sulfolobus acidocaldarius
Cl-
70 mM, 50% inhibition
Sulfolobus acidocaldarius
cyanide
1-3 mM, complete inhibition
Sulfolobus acidocaldarius
phosphate
23 mM, 50% inhibition
Sulfolobus acidocaldarius
SO42-
28 mM, 50% inhibition
Sulfolobus acidocaldarius
Sulfide
1-3 mM, complete inhibition
Sulfolobus acidocaldarius
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride
pH 7.0, 40°C
Sulfolobus acidocaldarius
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
the single subunit (38000-40000 Da) contains two copper ions
Sulfolobus acidocaldarius
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000
Sulfolobus acidocaldarius
100000
104000
gel filtration
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
caldariellaquinol + O2 + n H+/in
Sulfolobus acidocaldarius
-
caldariellaquinone + H2O + n H+/out
-
-
?
caldariellaquinol + O2 + n H+/in
Sulfolobus acidocaldarius DSM 639
-
caldariellaquinone + H2O + n H+/out
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36
-
pH 7.0, 40°C, oxidation of N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride
Sulfolobus acidocaldarius
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
caldariella quinol + O2 + n H+/in
isolated aa3 preparation does not oxidize cytochrome c
396010
Sulfolobus acidocaldarius
caldariella quinone + H2O + n H+/out
-
-
-
?
caldariella quinol + O2 + n H+/in
isolated aa3 preparation does not oxidize cytochrome c
396010
Sulfolobus acidocaldarius DSM 639
caldariella quinone + H2O + n H+/out
-
-
-
?
caldariellaquinol + O2 + n H+/in
-
396010
Sulfolobus acidocaldarius
caldariellaquinone + H2O + n H+/out
-
-
-
?
caldariellaquinol + O2 + n H+/in
-
396010
Sulfolobus acidocaldarius DSM 639
caldariellaquinone + H2O + n H+/out
-
-
-
?
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
396010
Sulfolobus acidocaldarius
?
-
-
-
?
N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
396010
Sulfolobus acidocaldarius DSM 639
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38000
Sulfolobus acidocaldarius
Other publictions for EC 7.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741970
Castelle
The aerobic respiratory chain ...
Ferroplasma acidiphilum
Biochim. Biophys. Acta
1847
717-728
2015
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-
-
-
-
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-
1
1
1
-
-
8
-
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1
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1
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1
1
1
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-
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-
-
-
-
-
-
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-
-
-
-
720065
Bandeiras
Structure and coordination of ...
Acidianus ambivalens
J. Biol. Inorg. Chem.
10
625-635
2005
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-
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-
-
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-
1
-
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2
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1
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1
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1
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1
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1
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656833
Muller
Coupling of the pathway of sul ...
Acidianus ambivalens
Mol. Microbiol.
53
1147-1160
2004
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1
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6
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1
1
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1
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1
1
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718705
Ishikawa
Aeropyrum pernix K1, a strictl ...
Aeropyrum pernix
Arch. Microbiol.
179
42-49
2002
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3
2
1
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3
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1
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1
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2
1
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2
1
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3
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2
1
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3
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1
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-
2
1
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2
1
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-
719024
Komorowski
The archaeal respiratory super ...
Sulfolobus acidocaldarius
Biol. Chem.
383
1791-1799
2002
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-
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-
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-
9
2
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2
1
1
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1
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3
1
1
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2
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1
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1
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9
-
2
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2
1
1
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-
-
3
1
1
-
-
-
2
-
-
-
-
1
1
-
-
-
718959
Gilderson
Kinetics of electron and proto ...
Acidianus ambivalens, Acidianus ambivalens DSM 3772
Biochim. Biophys. Acta
1503
261-270
2001
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1
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2
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4
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1
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4
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1
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1
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4
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1
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719770
Komorowski
First expression and character ...
Sulfolobus acidocaldarius
J. Bioenerg. Biomembr.
33
27-34
2001
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1
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1
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1
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1
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1
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1
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-
1
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724988
Komorowski
Sulfocyanin and subunit II, tw ...
Sulfolobus acidocaldarius
FEBS Lett.
487
351-355
2001
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1
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4
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1
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1
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1
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1
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724989
Gomes
Heme-copper oxidases with modi ...
Acidianus ambivalens
FEBS Lett.
497
159-164
2001
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2
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1
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720951
Das
Redox-linked transient deproto ...
Acidianus ambivalens
Proc. Natl. Acad. Sci. USA
96
9591-9596
1999
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719396
Giuffre
Functional properties of the q ...
Acidianus ambivalens
Eur. J. Biochem.
250
383-388
1997
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1
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4
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1
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719689
Purschke
The terminal quinol oxidase of ...
Acidianus ambivalens, Acidianus ambivalens DSM 3772
J. Bacteriol.
179
1344-1353
1997
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1
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3
1
1
2
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5
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2
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1
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2
1
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719793
Gleissner
The archaeal SoxABCD complex i ...
Sulfolobus acidocaldarius
J. Biol. Chem.
272
8417-8426
1997
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1
4
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1
2
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6
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4
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4
4
680598
Iwasaki
Resolution of the aerobic resp ...
Sulfolobus sp. 7, Sulfolobus sp.
J. Biol. Chem.
270
30893-30901
1995
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12
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722598
Iwasaki
Resolution of the aerobic resp ...
Sulfolobus sp. 7, Sulfolobus sp.
J. Biol. Chem.
270
30881-30892
1995
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1
1
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12
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679623
Gleissner
Generation of proton-motive fo ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
Eur. J. Biochem.
224
983-990
1994
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1
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2
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22
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1
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4
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4
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719394
Luebben
A second terminal oxidase in S ...
Sulfolobus acidocaldarius
Eur. J. Biochem.
224
151-159
1994
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1
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1
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1
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1
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719514
Anemüller
-
A cytochrome aa3-type quinol o ...
Acidianus ambivalens
FEMS Microbiol. Lett.
117
275-280
1994
-
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3
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1
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1
1
3
1
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6
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6
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