Cloned (Comment) | Organism |
---|---|
expression in Mycobacterium smegmatis | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
ubiquinone-2 | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.1 | - |
menaquinone | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.12 | - |
NADH | cosubstrate ubiquinone-2, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.14 | - |
NADH | cosubstrate ubiquinone-0, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.23 | - |
NADH | cosubstrate menaquinone, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.35 | - |
ubiquinone-0 | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes the transfer electrons from NADH to quinone substrates by a nonclassical, two-site pingpong kinetic mechanism whereby substrate quinones bind to a site that is distinct from the NADH-binding site. Presence of two binding sites for quinone ligands, one favoring the reduced form and the other favoring the oxidized form | Mycobacterium tuberculosis | ? | - |
? | |
NADH + H+ + menaquinone | - |
Mycobacterium tuberculosis | NAD+ + menaquinol | - |
? | |
NADH + H+ + ubiquinone-0 | - |
Mycobacterium tuberculosis | NAD+ + ubiquinol-0 | - |
? | |
NADH + H+ + ubiquinone-1 | - |
Mycobacterium tuberculosis | NAD+ + ubiquinol-1 | - |
? | |
NADH + H+ + ubiquinone-2 | - |
Mycobacterium tuberculosis | NAD+ + ubiquinol-2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDH-2 | - |
Mycobacterium tuberculosis |
type II NADH-quinone oxidoreductase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
423 | - |
menaquinone | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
480 | - |
ubiquinone-0 | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
640 | - |
ubiquinone-2 | pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis |