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Literature summary for 7.1.1.2 extracted from

  • Yano, T.; Rahimian, M.; Aneja, K.K.; Schechter, N.M.; Rubin, H.; Scott, C.P.
    Mycobacterium tuberculosis type II NADH-menaquinone oxidoreductase catalyzes electron transfer through a two-site ping-pong mechanism and has two quinone-binding sites (2014), Biochemistry, 53, 1179-1190.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Mycobacterium smegmatis Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
ubiquinone-2 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.1
-
menaquinone pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.12
-
NADH cosubstrate ubiquinone-2, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.14
-
NADH cosubstrate ubiquinone-0, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.23
-
NADH cosubstrate menaquinone, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.35
-
ubiquinone-0 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes the transfer electrons from NADH to quinone substrates by a nonclassical, two-site pingpong kinetic mechanism whereby substrate quinones bind to a site that is distinct from the NADH-binding site. Presence of two binding sites for quinone ligands, one favoring the reduced form and the other favoring the oxidized form Mycobacterium tuberculosis ?
-
?
NADH + H+ + menaquinone
-
Mycobacterium tuberculosis NAD+ + menaquinol
-
?
NADH + H+ + ubiquinone-0
-
Mycobacterium tuberculosis NAD+ + ubiquinol-0
-
?
NADH + H+ + ubiquinone-1
-
Mycobacterium tuberculosis NAD+ + ubiquinol-1
-
?
NADH + H+ + ubiquinone-2
-
Mycobacterium tuberculosis NAD+ + ubiquinol-2
-
?

Synonyms

Synonyms Comment Organism
NDH-2
-
Mycobacterium tuberculosis
type II NADH-quinone oxidoreductase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
423
-
menaquinone pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
480
-
ubiquinone-0 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
640
-
ubiquinone-2 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis