Cloned (Comment) | Organism |
---|---|
expression of His-tagged complex I | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe-S cluster, 29.0-0.37 of iron/1 mol of complex I | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
570000 | - |
about, recombinant His-tagged complex I, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ubiquinone + 6 H+[side 1] | Escherichia coli | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged complex I by nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ubiquinone + 6 H+[side 1] | - |
Escherichia coli | NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | complex I consists of 13 different subunits named NuoA-Nand encoded by the nuo operon | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
complex I | - |
Escherichia coli |
proton-translocating NADH:ubiquinone oxidoreductase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | 0.94-0.1 of FMN//1 mol of complex I | Escherichia coli | |
NADH | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | NADH:ubiquinone oxidoreductase (complex I) pumps protons across the membrane using downhill redox energy | Escherichia coli |