Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.2 extracted from

  • Birrell, J.A.; King, M.S.; Hirst, J.
    A ternary mechanism for NADH oxidation by positively charged electron acceptors, catalyzed at the flavin site in respiratory complex I (2011), FEBS Lett., 585, 2318-2322.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP-ribose
-
Bos taurus
ATP
-
Bos taurus
additional information NADH, ATP, ADP and ADP-ribose stimulate the enzyme reactions, indicating that the positively-charged acceptors interact with their negatively charged phosphates. In contrast to direct O2 reduction the rate is stimulated, not inhibited, by high NADH concentrations. When NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction. Adenosine, which lacks phosphates, does not stimulate the reaction with hexaammineruthenium(III), HAR, ternary mechanism of HAR and paraquat reduction,overview Bos taurus
NADH
-
Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
additional information when NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + ubiquinone + 6 H+[side 1] Bos taurus
-
NAD+ + ubiquinol + 7 H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the positively-charged electron acceptors paraquat and hexaammineruthenium(III) react with the nucleotide-bound reduced flavin in complex I, by an unusual ternary mechanism, overview. The mechanism for paraquat reduction defines another mechanism for superoxide production by complex I by redox cycling Bos taurus ?
-
?
NADH + ubiquinone + 6 H+[side 1]
-
Bos taurus NAD+ + ubiquinol + 7 H+[side 2]
-
?

Synonyms

Synonyms Comment Organism
complex I
-
Bos taurus
NADH:ubiquinone oxidoreductase
-
Bos taurus
respiratory complex I
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Bos taurus

General Information

General Information Comment Organism
physiological function the flavin mononucleotide in complex I catalyzes NADH oxidation, O2 reduction to superoxide, and the reduction of several artificial electron acceptors Bos taurus