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Literature summary for 7.1.1.2 extracted from

  • King, M.S.; Sharpley, M.S.; Hirst, J.
    Reduction of hydrophilic ubiquinones by the flavin in mitochondrial NADH:ubiquinone oxidoreductase (Complex I) and production of reactive oxygen species (2009), Biochemistry, 48, 2053-2062.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
 NADH pH 7.6, 32°C, cosubstrate: ubiquinone-1, ubiquinone-0 or idebenone Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a membrane-bound enzyme that contains 45 different subunits Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains eight iron-sulfur clusters Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + ubiquinone-10 Bos taurus the natural substrate ubiquinone-10 is extremely hydrophobic NAD+ + ubiquinol-10
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + decylubiquinone
-
 Bos taurus NAD+ + decylubiquinol
-
 ?
NADH + H+ + idebenone studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ?
-
 ?
NADH + H+ + ubiquinone-0
-
 Bos taurus NAD+ + ubiquinol-0
-
 ?
NADH + H+ + ubiquinone-1 studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ubiquinol-1
-
 ?
NADH + H+ + ubiquinone-10 the natural substrate ubiquinone-10 is extremely hydrophobic Bos taurus NAD+ + ubiquinol-10
-
 ?
NADH + H+ + ubiquinone-10 studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ubiquinol-10
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Bos taurus
NADH:ubiquinone oxidoreductase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 assay at Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 NADH pH 7.6, 32°C, cosubstrate: ubiquinone-1, ubiquinone-0 or idebenone Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
FMN contains non-covalently bound flavin mononucleotide. Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation Bos taurus
NADH
-
 Bos taurus